Epitopes described in "Predominance of HLA-restricted cytotoxic T-lymphocyte responses to serotype-cross-reactive epitopes on nonstructural proteins following natural secondary dengue virus infection."

Reference
Article Authors:A Mathew; I Kurane; S Green; H A Stephens; D W Vaughn; S Kalayanarooj; S Suntayakorn; D Chandanayingyong; F A Ennis; A L Rothman
Article Title:Predominance of HLA-restricted cytotoxic T-lymphocyte responses to serotype-cross-reactive epitopes on nonstructural proteins following natural secondary dengue virus infection.
Reference Detail
Reference ID:1000440
Abstract:We examined the memory cytotoxic T-lymphocytic (CTL) responses of peripheral blood mononuclear cells (PBMC) obtained from patients in Thailand 12 months after natural symptomatic secondary dengue virus infection. In all four patients analyzed, CTLs were detected in bulk culture PBMC against nonstructural dengue virus proteins. Numerous CD4+ and CD8+ CTL lines were generated from the bulk cultures of two patients, KPP94-037 and KPP94-024, which were specific for NS1.2a (NS1 and NS2a collectively) and NS3 proteins, respectively. All CTL lines derived from both patients were cross-reactive with other serotypes of dengue virus. The CD8+ NS1.2a-specific lines from patient KPP94-037 were HLA B57 restricted, and the CD8+ NS3-specific lines from patient KPP94-024 were HLA B7 restricted. The CD4+ CTL lines from patient KPP94-037 were HLA DR7 restricted. A majority of the CD8+ CTLs isolated from patient KPP94-024 were found to recognize amino acids 221 to 232 on NS3. These results demonstrate that in Thai patients after symptomatic secondary natural dengue infections, CTLs are mainly directed against nonstructural proteins and are broadly cross-reactive.
Date:1998
Reference Type:Literature
PubMed ID:9557687
Journal:J Virol
Journal Volume:72
Article Pages:3999-4004
Journal ISSN:1098-5514
Article Chemical List:Epitopes, T-Lymphocyte;HLA Antigens;NS1 protein, Dengue virus type 2;NS2A protein, Dengue virus type 2;NS3 protein, flavivirus;Viral Nonstructural Proteins;Serine Endopeptidases;RNA Helicases
Article MeSH List:CD4-Positive T-Lymphocytes(immunology); CD8-Positive T-Lymphocytes(immunology); Cells, Cultured; Child; Cross Reactions; Dengue(immunology); Dengue Virus(immunology); Epitope Mapping; Epitopes, T-Lymphocyte(immunology); HLA Antigens(immunology); Humans; Leukocytes, Mononuclear(immunology); RNA Helicases; Serine Endopeptidases; Serotyping; T-Lymphocytes, Cytotoxic(immunology); Thailand; Viral Nonstructural Proteins(immunology)
Curation Last Updated:2014-04-01 18:10:51