Epitopes described in "Production of recombinant virus-like particles from human papillomavirus types 6 and 11, and study of serological reactivities between HPV 6, 11, 16 and 45 by ELISA: implications for papillomavirus prevention and detection."

Reference
Article Authors:A Touzé; C Dupuy; D Mahé; P Y Sizaret; P Coursaget
Article Title:Production of recombinant virus-like particles from human papillomavirus types 6 and 11, and study of serological reactivities between HPV 6, 11, 16 and 45 by ELISA: implications for papillomavirus prevention and detection.
Reference Detail
Reference ID:1006105
Abstract:The L1 major capsid proteins of human papillomaviruses types 6 and 11 were expressed in insect cells using recombinant baculoviruses. These L1 proteins were shown to self-assemble into virus-like particles resembling papillomavirus virions as previously observed for HPV 16 and 45. However, we observed variations in the yield of virus-like particles among the four genotypes investigated. This suggests that more than one strain of each genotype has to be investigated to obtain the high level of virus-like particle production necessary to develop HPV vaccines or serological tests. Cross-reactivities between HPV 6, 11, 16 and 45 were studied using polyclonal and monoclonal antibodies to virus-like particles, L1 proteins and synthetic peptides. Although antisera react strongly against homologous virus-like particles, there is evidence of some cross-reactivity. This could be one of the explanations for the fact that antibodies to one genotype are detected in individuals infected with another genotype. This study also identified a linear epitope recognized by anti-HPV 16 virus-like particle sera.
Affiliations:Laboratoire d'Immunologie des Maladies Infectieuses, Faculté des Sciences Pharmaceutiques Philippe Maupas, Tours, France.
Date:1998
Reference Type:Literature
PubMed ID:9495021
Journal:FEMS Microbiol Lett
Journal Volume:160
Article Pages:111-8
Journal ISSN:0378-1097
Article Chemical List:Antibodies, Monoclonal;Viral Envelope Proteins;Viral Fusion Proteins;NEG envelope glycoprotein, Negishi virus
Article MeSH List:Amino Acid Sequence; Animals; Antibodies, Monoclonal; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Genes, Viral(genetics ); Genotype; Molecular Sequence Data; Papillomaviridae(genetics; immunology; isolation & purification ); Papillomavirus Infections(diagnosis; prevention & control ); Spodoptera; Tumor Virus Infections(diagnosis; prevention & control ); Viral Envelope Proteins(genetics; immunology ); Viral Fusion Proteins(genetics; immunology ); Virion(isolation & purification )
Curation Last Updated:2014-07-16 21:13:43