Epitopes described in "Synthetic peptides derived from the melanocyte-stimulating hormone receptor MC1R can stimulate HLA-A2-restricted cytotoxic T lymphocytes that recognize naturally processed peptides on human melanoma cells."

Reference
Article Authors:F Salazar-Onfray; T Nakazawa; V Chhajlani; M Petersson; K Kärre; G Masucci; E Celis; A Sette; S Southwood; E Appella; R Kiessling
Article Title:Synthetic peptides derived from the melanocyte-stimulating hormone receptor MC1R can stimulate HLA-A2-restricted cytotoxic T lymphocytes that recognize naturally processed peptides on human melanoma cells.
Reference Detail
Reference ID:200075
Abstract:Human melanoma-specific HLA-A2 restricted CTLs have recently been shown to recognize antigens expressed by melanoma lines and normal melanocytes, including Melan-A/Mart-1, gp100, gp75, and tyrosinase. Herein, we define HLA-A2-restricted CTL epitopes from a recently cloned melanocortin 1 receptor (MC1R), which belongs to a new subfamily of the G-protein-coupled receptors expressed on melanomas and melanocytes. Thirty-one MC1R-derived peptides were selected on the basis of HLA-A2-specific motifs and tested for their HLA-A2 binding capacity. Of a group of 12 high or intermediate HLA-A2 binding peptides, three nonamers, MC1R244 (TILLGIFFL), MC1R283 (FLALIICNA), and MC1R291 (AIIDPLIYA), were found to induce peptide-specific CTLs from peripheral blood mononuclear cells of healthy HLA-A2+ donors after repeated in vitro stimulation with peptide-pulsed antigen-presenting cells. The CTLs raised against these three HLA-A2+-restricted peptides could recognize naturally processed peptides from HLA-A2+ melanomas and from Cos7 cells cotransfected with MC1R and HLA-A2. CTLs induced by the MC1R291 peptide (but not induced or induced only to a very low extent by the other two MCR1 peptide epitopes) showed cross-reactions with two other members of the melanocortin receptor family, which are more broadly expressed on other tissues. Taken together, our findings have implications in relation both to autoimmunity and immunotherapy of malignant melanomas.
Affiliations:Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
Date:1997
Reference Type:Literature
PubMed ID:9331097
Journal:Cancer Res
Journal Volume:57
Article Pages:4348-55
Journal ISSN:1538-7445
Article Chemical List:Antigens, Neoplasm;HLA-A2 Antigen;Peptide Fragments;Receptors, Corticotropin;Receptors, Melanocortin
Article MeSH List:Animals; Antigen Presentation; Antigens, Neoplasm(chemistry; immunology); Autoimmunity; COS Cells; HLA-A2 Antigen(immunology); Humans; Immunotherapy; Melanoma(immunology; pathology); Peptide Fragments(chemical synthesis; pharmacology); Receptors, Corticotropin(chemistry); Receptors, Melanocortin; T-Lymphocytes, Cytotoxic(drug effects); Tumor Cells, Cultured
Article Comments:Data originally imported from the Database of Functional Molecular Immunology, FIMM (http://sdmc.lit.org.sg:8080/fimm/) and from the HLA Ligand Database (http://hlaligand.ouhsc.edu/)
Curation Last Updated:2014-03-26 11:57:06