Epitopes described in "Rapid effector function in CD8+ memory T cells."

Article Authors:A Lalvani; R Brookes; S Hambleton; W J Britton; A V Hill; A J McMichael
Article Title:Rapid effector function in CD8+ memory T cells.
Reference Detail
Reference ID:1465
Abstract:The nature of the CD8+ T cells that underlie antiviral protective immunological memory in vivo is unclear. We have characterized peptide-specific CD8+ T lymphocytes directly ex vivo from peripheral blood in humans with past exposure to influenza virus, using single cell interferon gamma (IFN-gamma) release as a measure of effector function. In individuals in the memory state with respect to influenza virus infection, unrestimulated antigen-specific CD8+ T cells displayed IFN-gamma release within 6 h of antigen contact, identifying a population of memory CD8+ T cells that exhibit effector function without needing to divide and differentiate over several days. We have quantified circulating CD8+ effector T cells specific for six different MHC class I-restricted influenza virus epitopes. Enumeration of these CD8+ T cells gives frequencies of peptide-specific T cells that correlate with, but are in general severalfold higher than, CTL precursor frequencies derived from limiting dilution analysis, indicating that this novel population of memory CD8+ T cells has hitherto been undetected by standard means. The phenotype of these cells, which persist at a low frequency long after recovery from an acute viral infection, suggests that they play a role in protective immunological memory.
Affiliations:Molecular Immunology Group, Institute of Molecular Medicine, Nuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom. ajit.lalvani@ndm.ox.ac.uk.
Reference Type:Literature
PubMed ID:9294140
Journal:J Exp Med
Journal Volume:186
Article Pages:859-65
Journal ISSN:0022-1007
Article Chemical List:Antigens, Viral;Epitopes;HLA Antigens;Histocompatibility Antigens Class I;Receptors, Antigen, T-Cell, alpha-beta;Interferon-gamma
Article MeSH List:Adult; Amino Acid Sequence; Antigens, Viral(administration & dosage; genetics); CD8-Positive T-Lymphocytes(immunology); Epitopes(administration & dosage; genetics); HLA Antigens(metabolism); Histocompatibility Antigens Class I(metabolism); Humans; Immunologic Memory; In Vitro Techniques; Influenza, Human(immunology); Interferon-gamma(secretion); Orthomyxoviridae(genetics; immunology); Phenotype; Receptors, Antigen, T-Cell, alpha-beta(metabolism); T-Lymphocytes, Cytotoxic(immunology)
Curation Last Updated:2016-01-08 20:05:39