Epitopes described in "Phenotypic analysis of antigen-specific T lymphocytes."

Article Authors:J D Altman; P A Moss; P J Goulder; D H Barouch; M G McHeyzer-Williams; J I Bell; A J McMichael; M M Davis
Article Title:Phenotypic analysis of antigen-specific T lymphocytes.
Reference Detail
Reference ID:1000181
Abstract:Identification and characterization of antigen-specific T lymphocytes during the course of an immune response is tedious and indirect. To address this problem, the peptide-major histocompatability complex (MHC) ligand for a given population of T cells was multimerized to make soluble peptide-MHC tetramers. Tetramers of human lymphocyte antigen A2 that were complexed with two different human immunodeficiency virus (HIV)-derived peptides or with a peptide derived from influenza A matrix protein bound to peptide-specific cytotoxic T cells in vitro and to T cells from the blood of HIV-infected individuals. In general, tetramer binding correlated well with cytotoxicity assays. This approach should be useful in the analysis of T cells specific for infectious agents, tumors, and autoantigens.
Affiliations:Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5428, USA.
Reference Type:Literature
PubMed ID:8810254
Journal Volume:274
Article Pages:94-6
Journal ISSN:1095-9203
Article Chemical List:Antigens, Viral;Coloring Agents;Epitopes;Gene Products, gag;HLA-A2 Antigen;Peptide Fragments;Viral Matrix Proteins;influenza virus membrane protein (58-66);RNA-Directed DNA Polymerase
Article MeSH List:Amino Acid Sequence; Antigens, Viral(immunology); Base Sequence; CD8-Positive T-Lymphocytes(immunology); Cell Line; Coloring Agents; Epitopes(immunology); Flow Cytometry; Gene Products, gag(immunology); HIV Seropositivity(immunology); HLA-A2 Antigen(immunology); Humans; Molecular Sequence Data; Peptide Fragments(immunology); Phenotype; RNA-Directed DNA Polymerase(immunology); T-Lymphocytes, Cytotoxic(immunology); Viral Matrix Proteins(immunology)
Article Comments:ErratumIn(Science 1998 Jun 19;280(5371):1821 )
Curation Last Updated:2014-10-03 19:46:51