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| Article Authors: | J D Altman; P A Moss; P J Goulder; D H Barouch; M G McHeyzer-Williams; J I Bell; A J McMichael; M M Davis |
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| Article Title: | Phenotypic analysis of antigen-specific T lymphocytes. |
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| Reference ID: | 1000181 |
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| Abstract: | Identification and characterization of antigen-specific T lymphocytes during the course of an immune response is tedious and indirect. To address this problem, the peptide-major histocompatability complex (MHC) ligand for a given population of T cells was multimerized to make soluble peptide-MHC tetramers. Tetramers of human lymphocyte antigen A2 that were complexed with two different human immunodeficiency virus (HIV)-derived peptides or with a peptide derived from influenza A matrix protein bound to peptide-specific cytotoxic T cells in vitro and to T cells from the blood of HIV-infected individuals. In general, tetramer binding correlated well with cytotoxicity assays. This approach should be useful in the analysis of T cells specific for infectious agents, tumors, and autoantigens. |
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| Affiliations: | Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5428, USA. |
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| Date: | 1996 |
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| Reference Type: | Literature |
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| PubMed ID: | 8810254 |
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| Journal: | Science |
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| Journal Volume: | 274 |
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| Article Pages: | 94-6 |
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| Journal ISSN: | 1095-9203 |
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| Article Chemical List: | Antigens, Viral;Coloring Agents;Epitopes;Gene Products, gag;HLA-A2 Antigen;Peptide Fragments;Viral Matrix Proteins;influenza virus membrane protein (58-66);RNA-Directed DNA Polymerase |
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| Article MeSH List: | Amino Acid Sequence; Antigens, Viral(immunology); Base Sequence; CD8-Positive T-Lymphocytes(immunology); Cell Line; Coloring Agents; Epitopes(immunology); Flow Cytometry; Gene Products, gag(immunology); HIV Seropositivity(immunology); HLA-A2 Antigen(immunology); Humans; Molecular Sequence Data; Peptide Fragments(immunology); Phenotype; RNA-Directed DNA Polymerase(immunology); T-Lymphocytes, Cytotoxic(immunology); Viral Matrix Proteins(immunology) |
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| Article Comments: | ErratumIn(Science 1998 Jun 19;280(5371):1821 ) |
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| Curation Last Updated: | 2011-10-24 20:39:09 |
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