Epitopes described in "Protection of mice against influenza A virus challenge by vaccination with baculovirus-expressed M2 protein."

Article Authors:V A Slepushkin; J M Katz; R A Black; W C Gamble; P A Rota; N J Cox
Article Title:Protection of mice against influenza A virus challenge by vaccination with baculovirus-expressed M2 protein.
Reference Detail
Reference ID:1019185
Abstract:We have investigated the potential of the conserved transmembrane M2 protein of influenza A/Ann Arbor/6/60 virus, expressed by a baculovirus recombinant, to induce protective immunity in BALB/c mice. Vaccination of mice with M2 shortened the duration of virus shedding and protected mice from a lethal infection with A/Ann Arbor/6/60 virus but not B/Ann Arbor/1/55 virus, suggesting that the protection was mediated by an M2-specific mechanism. Serum antibodies were detected which reacted with synthetic peptides defining three antigenic determinants located on both the external N- and internal C-termini of the M2 protein. Furthermore, vaccination with M2 protected mice from death following a lethal challenge with the heterologous A/Hong Kong/68 (H3N2) virus. These results demonstrate the potential to elicit heterosubtypic immunity to type A influenza viruses through vaccination with a conserved transmembrane protein.
Affiliations:Influenza Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Reference Type:Literature
PubMed ID:8578816
Journal Volume:13
Article Pages:1399-402
Journal ISSN:0264-410X
Article Chemical List:Antibodies, Viral;Influenza Vaccines;M-protein, influenza virus;M2 protein, Influenza A virus;Recombinant Proteins;Vaccines, Synthetic;Viral Matrix Proteins
Article MeSH List:Amino Acid Sequence; Animals; Antibodies, Viral(biosynthesis); Antibody Specificity; Baculoviridae(genetics); Female; Genetic Vectors(immunology); Influenza A virus(immunology); Influenza Vaccines(immunology); Mice; Mice, Inbred BALB C; Molecular Sequence Data; Orthomyxoviridae Infections(prevention & control; virology); Recombinant Proteins(biosynthesis; immunology); Vaccines, Synthetic(immunology); Viral Matrix Proteins(genetics; immunology)
Curation Last Updated:2014-10-03 21:58:50