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| Article Authors: | M E Ressing; W J van Driel; E Celis; A Sette; M P Brandt; M Hartman; J D Anholts; G M Schreuder; W B ter Harmsel; G J Fleuren; B J Trimbos; W M Kast; C J Melief |
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| Article Title: | Occasional memory cytotoxic T-cell responses of patients with human papillomavirus type 16-positive cervical lesions against a human leukocyte antigen-A *0201-restricted E7-encoded epitope. |
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| Reference ID: | 1000256 |
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| Abstract: | Most cervical carcinoma (Cxca) cells constitutively express human papillomavirus type 16 (HPV16) E6 and E7 oncoproteins. These proteins are, therefore, attractive targets for T cell-based immunotherapy. Previously, we identified HVP16 E7-encoded CTL epitopes. In patients with cervical intraepithelial neoplasia or Cxca, little is known concerning T-cell activity against viruses in general and against HPV16 in particular. Here, we have screened the blood of 10 healthy donor controls and of 22 patients with HPV16+ cervical lesions for the presence of CTLs directed against HPV16 E7- and control influenza virus matrix-derived epitopes presented by HLA-A *0201. We detected influenza virus-specific CTLs in all donors and in the majority of patients, indicating that most patients have functioning T-cell responses despite their lesions or therapeutic interventions. Moreover, we show that patients with HPV16+ lesions occasionally have memory CTLs against a HPV16 E7-encoded epitope (sequence YMLD-LQPETT), providing evidence for natural CTL immunity against HPV16 in patients with cervical lesions. Combined, these findings raise possibilities for vaccination with HPV16 E7-encoded peptides to induce or augment CTL responses for treatment or prevention of Cxca. |
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| Affiliations: | Department of Immunohematology, University Hospital Leiden, The Netherlands. |
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| Date: | 1996 |
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| Reference Type: | Literature |
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| PubMed ID: | 8564975 |
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| Journal: | Cancer Res |
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| Journal Volume: | 56 |
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| Article Pages: | 582-8 |
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| Journal ISSN: | 1538-7445 |
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| Article Chemical List: | Epitopes;HLA-A Antigens;Oncogene Proteins, Viral;Papillomavirus E7 Proteins;oncogene protein E7, Human papillomavirus type 16 |
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| Article MeSH List: | Amino Acid Sequence; Cervical Intraepithelial Neoplasia(immunology; virology); Epitopes(immunology); Female; HLA-A Antigens(immunology); Humans; Immunologic Memory(immunology); Influenza A virus(immunology); Molecular Sequence Data; Oncogene Proteins, Viral(immunology); Papillomaviridae(immunology); Papillomavirus E7 Proteins; T-Lymphocytes, Cytotoxic(immunology); Uterine Cervical Neoplasms(immunology; virology) |
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| Curation Last Updated: | 2013-01-11 10:31:26 |
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