Epitopes described in "Structural requirements for peptides that stimulate a subset of gamma delta T cells."

Article Authors:Y X Fu; M Vollmer; H Kalataradi; K Heyborne; C Reardon; C Miles; R O'Brien; W Born
Article Title:Structural requirements for peptides that stimulate a subset of gamma delta T cells.
Reference Detail
Reference ID:1003665
Abstract:Hybridomas representing the V gamma 1-positive subset of murine gamma delta T cells secrete lymphokines in response to synthetic peptides representing a short segment of the mycobacterial 60-kDa heat shock protein (HSP-60). Here we show the TCR dependency of this response by transfection of productively rearranged TCR genes derived from an HSP-60 reactive gamma delta T cell hybridoma. We also have defined structural requirements for the stimulatory peptide. The smallest HSP-60 peptide capable of stimulating these hybridomas is seven amino acids long, representing positions 181-187, and having the sequence FGLQLEL. Amino acid-substituted derivatives of this peptide, and another containing the same core, p180-190, revealed amino acids essential for stimulatory activity. Phenylalanine in position 181 and leucine in position 183 seem to be required for stimulation of all HSP-60 reactive cells, whereas others are only required by some. Clonal differences in the responses to these peptides provide indirect evidence for cognate TCR-peptide interactions. The smallest stimulatory peptide, p181-187, represents an area not well conserved among HSP-60 molecules of other species, and stimulates a mycobacteria-specific response unlike the earlier observed cross-reactive responses of the same hybridomas with longer HSP-60 peptides derived from mycobacteria and other species (our manuscript in preparation). We propose that the TCR-dependent multiclonal gamma delta T cell response to HSP-60 peptides and derivatives, which in some ways resembles superantigen responses and in other ways resembles responses to conventional Ag, may be a separate, third type of Ag response by T cells.
Affiliations:Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
Reference Type:Literature
PubMed ID:8120371
Journal:J Immunol
Journal Volume:152
Article Pages:1578-88
Journal ISSN:0022-1767
Article Chemical List:Heat-Shock Proteins;Peptide Fragments;Receptors, Antigen, T-Cell, gamma-delta
Article MeSH List:Amino Acid Sequence; Animals; Base Sequence; Heat-Shock Proteins(immunology ); Lymphocyte Activation; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Peptide Fragments(immunology ); Receptors, Antigen, T-Cell, gamma-delta(analysis ); Structure-Activity Relationship; T-Lymphocyte Subsets(immunology )
Curation Last Updated:2015-07-30 20:15:01