|
| Article Authors: | Y X Fu; M Vollmer; H Kalataradi; K Heyborne; C Reardon; C Miles; R O'Brien; W Born |
|---|
| Article Title: | Structural requirements for peptides that stimulate a subset of gamma delta T cells. |
|---|
|
| Reference ID: | 1003665 |
|---|
| Abstract: | Hybridomas representing the V gamma 1-positive subset of murine gamma delta T cells secrete lymphokines in response to synthetic peptides representing a short segment of the mycobacterial 60-kDa heat shock protein (HSP-60). Here we show the TCR dependency of this response by transfection of productively rearranged TCR genes derived from an HSP-60 reactive gamma delta T cell hybridoma. We also have defined structural requirements for the stimulatory peptide. The smallest HSP-60 peptide capable of stimulating these hybridomas is seven amino acids long, representing positions 181-187, and having the sequence FGLQLEL. Amino acid-substituted derivatives of this peptide, and another containing the same core, p180-190, revealed amino acids essential for stimulatory activity. Phenylalanine in position 181 and leucine in position 183 seem to be required for stimulation of all HSP-60 reactive cells, whereas others are only required by some. Clonal differences in the responses to these peptides provide indirect evidence for cognate TCR-peptide interactions. The smallest stimulatory peptide, p181-187, represents an area not well conserved among HSP-60 molecules of other species, and stimulates a mycobacteria-specific response unlike the earlier observed cross-reactive responses of the same hybridomas with longer HSP-60 peptides derived from mycobacteria and other species (our manuscript in preparation). We propose that the TCR-dependent multiclonal gamma delta T cell response to HSP-60 peptides and derivatives, which in some ways resembles superantigen responses and in other ways resembles responses to conventional Ag, may be a separate, third type of Ag response by T cells. |
|---|
| Affiliations: | Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206. |
|---|
| Date: | 1994 |
|---|
| Reference Type: | Literature |
|---|
| PubMed ID: | 8120371 |
|---|
| Journal: | J Immunol |
|---|
| Journal Volume: | 152 |
|---|
| Article Pages: | 1578-88 |
|---|
| Journal ISSN: | 0022-1767 |
|---|
| Article Chemical List: | Heat-Shock Proteins;Peptide Fragments;Receptors, Antigen, T-Cell, gamma-delta |
|---|
| Article MeSH List: | Amino Acid Sequence; Animals; Base Sequence; Heat-Shock Proteins(immunology ); Lymphocyte Activation; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Peptide Fragments(immunology ); Receptors, Antigen, T-Cell, gamma-delta(analysis ); Structure-Activity Relationship; T-Lymphocyte Subsets(immunology ) |
|---|
| Curation Last Updated: | 2013-05-28 20:43:19 |
|---|
