Epitopes described in "Visualization of A beta 42(43) and A beta 40 in senile plaques with end-specific A beta monoclonals: evidence that an initially deposited species is A beta 42(43)."

Reference
Article Authors:T Iwatsubo; A Odaka; N Suzuki; H Mizusawa; N Nukina; Y Ihara
Article Title:Visualization of A beta 42(43) and A beta 40 in senile plaques with end-specific A beta monoclonals: evidence that an initially deposited species is A beta 42(43).
Reference Detail
Reference ID:1008368
Abstract:To learn about the carboxy-terminal extent of amyloid beta-protein (A beta) composition of senile plaques (SPs) in the brain affected with Alzheimer's disease (AD), we employed two end-specific monoclonal antibodies as immunocytochemical probes: one is specific for A beta 40, the carboxyl terminus of A beta 1-40, while the other is specific for A beta 42(43). In the AD cortex, all SPs that were labeled with an authentic antibody were A beta 42(43) positive, while only one-third of which, on the average, were A beta 40 positive. There was a strong correlation between A beta 40 positivity and mature plaques. Two familial AD cortices with the mutation of beta-amyloid protein precursor 717 (beta APP717) (Val to Ile) showed a remarkable predominance of A beta 42(43)-positive, A beta 40-negative plaques. Diffuse plaques, representing the earliest stage of A beta deposition, were exclusively positive for A beta 42(43), but completely negative for A beta 40.
Affiliations:Department of Neuropathology and Neuroscience, Faculty of Pharmaceutical Sciences, Ibaraki, Japan.
Date:1994
Reference Type:Literature
PubMed ID:8043280
Journal:Neuron
Journal Volume:13
Article Pages:45-53
Journal ISSN:0896-6273
Article Chemical List:Amyloid beta-Peptides;Antibodies, Monoclonal;Peptide Fragments
Article MeSH List:Adult; Aged; Aged, 80 and over; Alzheimer Disease(genetics; metabolism; pathology); Amyloid beta-Peptides(chemistry; genetics; metabolism); Antibodies, Monoclonal; Brain(metabolism; pathology); Cerebral Cortex(chemistry; metabolism); Female; Humans; Immunoenzyme Techniques; Male; Middle Aged; Mutation; Peptide Fragments(analysis; immunology)
Curation Last Updated:2013-05-28 21:17:11