Epitopes described in "Development of high potency universal DR-restricted helper epitopes by modification of high affinity DR-blocking peptides."

Article Authors:J Alexander; J Sidney; S Southwood; J Ruppert; C Oseroff; A Maewal; K Snoke; H M Serra; R T Kubo; A Sette; et al.
Article Title:Development of high potency universal DR-restricted helper epitopes by modification of high affinity DR-blocking peptides.
Reference Detail
Reference ID:200044
Abstract:Pan DR-binding peptides engineered by introducing anchor residues for different DR motifs within a polyalanine backbone bound 10 of 10 DR molecules tested, with affinities, in most cases, in the nanomolar range. Because of the small methyl group exposed for T cell recognition, these peptides were poor immunogens but effective blockers of DR-restricted antigen presentation. Introduction of bulky and charged residues at positions accessible for T cell recognition yielded extremely powerful Pan DR epitope peptides (PADRE). These peptides elicited powerful responses in vitro from human peripheral blood mononuclear cells (PBMC). Because these cells also cross-react on certain mouse class II alleles, we could also demonstrate that PADRE peptides are active in vivo. In one example of their capacity to elicit T help, they were approximately 1000 times more powerful than natural T cell epitopes. We propose that PADRE peptides may be useful in the development of subunit vaccines.
Affiliations:Cytel Corporation, San Diego, California 92121.
Reference Type:Literature
PubMed ID:7895164
Journal Volume:1
Article Pages:751-61
Journal ISSN:1074-7613
Article Chemical List:HLA-DR Antigens;Peptides
Article MeSH List:Alleles; Amino Acid Sequence; Animals; Antigen Presentation; Binding Sites; Cell Division; Cross Reactions; HLA-DR Antigens(genetics; immunology ); Humans; Leukocytes, Mononuclear(immunology ); Lymphocyte Activation; Mice; Molecular Sequence Data; Peptides(chemical synthesis; immunology ); T-Lymphocytes, Helper-Inducer(cytology; immunology )
Curation Last Updated:2016-01-19 20:01:31