Epitopes described in "CD8+ T-cell protective immunity induced by immunization with Plasmodium berghei CS protein-derived synthetic peptides: evidence that localization of peptide-specific CTLs is crucial for protection against malaria."

Reference
Article Authors:J Renggli; D Valmori; J F Romero; G Eberl; P Romero; B Betschart; G Corradin
Article Title:CD8+ T-cell protective immunity induced by immunization with Plasmodium berghei CS protein-derived synthetic peptides: evidence that localization of peptide-specific CTLs is crucial for protection against malaria.
Reference Detail
Reference ID:1002922
Abstract:Immunization of BALB/c mice (H-2d) with a mixture of major histocompatibility complex (MHC) class I- and MHC class II-restricted synthetic peptides emulsified in incomplete Freund's adjuvant (IFA) induced a high level of specific cytotoxic T lymphocyte (CTL) activity. Peptides 249-260 or 252-260, derived from the circumsporozoite protein of Plasmodium berghei and representing a H-2Kd-restricted CTL epitope, were injected twice subcutaneously or intraperitoneally in BALB/c mice in combination with the tetanus toxin-derived universal T-helper peptide P30 in IFA. No protection was observed after exposure of immunized mice to infected mosquitoes. In contrast, when peptide 252-260-specific CTLs were expanded in vitro and adoptively transferred into naive recipient, mice were partially protected (64%) against a subsequent sporozoite challenge. Furthermore, direct transfer of lymph nodes or spleen cells from mice immunized with the peptide PbCS 252-260 also conferred protection to recipient mice. This protection was long-lasting and similar to that obtained with irradiated sporozoites.
Affiliations:Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
Date:1995
Reference Type:Literature
PubMed ID:7590920
Journal:Immunol Lett
Journal Volume:46
Article Pages:199-205
Journal ISSN:0165-2478
Article Chemical List:Epitopes;Peptides;Protozoan Proteins;circumsporozoite protein, Protozoan
Article MeSH List:Amino Acid Sequence; Animals; Epitopes; Female; Immunity, Cellular; Malaria(immunology; prevention & control ); Mice; Mice, Inbred BALB C; Molecular Sequence Data; Peptides(chemical synthesis; immunology ); Plasmodium berghei(immunology ); Protozoan Proteins(immunology ); T-Lymphocytes, Cytotoxic(immunology )
Curation Last Updated:2013-05-28 20:36:55