Epitopes described in "Amyloid-like properties of peptides flanking the epitope of amyloid precursor protein-specific monoclonal antibody 22C11."

Article Authors:C Hilbich; U Mönning; C Grund; C L Masters; K Beyreuther
Article Title:Amyloid-like properties of peptides flanking the epitope of amyloid precursor protein-specific monoclonal antibody 22C11.
Reference Detail
Reference ID:1011870
Abstract:Alzheimer's disease is one of the prevalent forms of human dementia. Its pathology is distinguished by proteinaceous deposits ("amyloid") in the brain. They contain a peptide (beta A4) that is proteolytically derived from a larger transmembrane protein. To follow the different metabolic pathways of this Amyloid Precursor Protein (APP) may thus lead to the elucidation of the molecular basis of Alzheimer's disease. Specific antibodies are necessary tools for this task. Using synthetic peptides, we have characterized the epitope of the APP-specific monoclonal antibody 22C11; it is localized between residues 66 and 81 of APP. Some of the peptides flanking this site exhibited properties generally associated with amyloid, i.e. low solubility, filament formation, and birefringence after Congo Red staining. Exploiting differences in the peptides' aggregational properties, we present evidence that the two dyes Eosin and Direct Red 254, in conjunction with classical amyloid staining by Congo Red, can be used to characterize aggregating, amyloid-like peptides in vitro.
Affiliations:Center of Molecular Biology, University of Heidelberg, Federal Republic of Germany.
Reference Type:Literature
PubMed ID:7504673
Journal:J Biol Chem
Journal Volume:268
Article Pages:26571-7
Journal ISSN:0021-9258
Article Chemical List:Amyloid;Amyloid beta-Protein Precursor;Antibodies, Monoclonal;Epitopes;Peptides
Article MeSH List:Amino Acid Sequence; Amyloid(metabolism ); Amyloid beta-Protein Precursor(immunology; metabolism ); Antibodies, Monoclonal(immunology; metabolism ); Epitopes(immunology ); Humans; Microscopy, Electron; Molecular Sequence Data; Peptides(chemical synthesis; immunology; metabolism )
Curation Last Updated:2014-10-03 21:08:49