Epitopes described in "Plant virus expressing hybrid coat protein with added murine epitope elicits autoantibody response."

Article Authors:J Fitchen; R N Beachy; M B Hein
Article Title:Plant virus expressing hybrid coat protein with added murine epitope elicits autoantibody response.
Reference Detail
Reference ID:1017052
Abstract:A modified tobacco mosaic virus (TMV) was used to direct the synthesis of TMV coat protein hybrids containing a 13 amino acid sequence of the murine zona pellucida ZP3 protein. This hybrid protein was found to be robust and accumulate to high concentrations in inoculated plants. Virus-like particles containing the hybrid coat protein could be readily purified from infected plant tissue. Parenteral immunization with these virus-like particles as antigen resulted in serum antibody recognizing ZP3 epitope in C57BL/6J and BALB/cBy mice. The antibodies recognized synthetic ZP3 peptide and the authentic murine ZP3 glycoprotein. Antibodies were recruited in vivo to the zone pellucida in female mice.
Affiliations:Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA.
Reference Type:Literature
PubMed ID:7491811
Journal Volume:13
Article Pages:1051-7
Journal ISSN:0264-410X
Article Chemical List:Antigens;Autoantibodies;Egg Proteins;Epitopes;Membrane Glycoproteins;Receptors, Cell Surface;Recombinant Fusion Proteins;Vaccines, Synthetic;zona pellucida glycoproteins
Article MeSH List:Animals; Antigens(immunology); Autoantibodies(blood); Base Sequence; Capsid(immunology); Egg Proteins(immunology); Epitopes; Female; Immunization; Membrane Glycoproteins(immunology); Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Molecular Sequence Data; Receptors, Cell Surface; Recombinant Fusion Proteins(immunology); Tobacco Mosaic Virus(genetics); Vaccines, Synthetic(immunology); Zona Pellucida(immunology)
Curation Last Updated:2014-10-03 21:49:07