Epitopes described in "High-throughput identification and dendritic cell-based functional validation of MHC class I-restricted Mycobacterium tuberculosis epitopes."

Reference
Article Authors:Smita K Nair; Georgia D Tomaras; Ana Paula Sales; David Boczkowski; Cliburn Chan; Kelly Plonk; Yongting Cai; Jens Dannull; Thomas B Kepler; Scott K Pruitt; Kent J Weinhold
Article Title:High-throughput identification and dendritic cell-based functional validation of MHC class I-restricted Mycobacterium tuberculosis epitopes.
Reference Detail
Reference ID:1027586
Abstract:Emergence of drug-resistant strains of the pathogen Mycobacterium tuberculosis (Mtb) and the ineffectiveness of BCG in curtailing Mtb infection makes vaccine development for tuberculosis an important objective. Identifying immunogenic CD8+ T cell peptide epitopes is necessary for peptide-based vaccine strategies. We present a three-tiered strategy for identifying and validating immunogenic peptides: first, identify peptides that form stable complexes with class I MHC molecules; second, determine whether cytotoxic T lymphocytes (CTLs) raised against the whole protein antigen recognize and lyse target cells pulsed with peptides that passed step 1; third, determine whether peptides that passed step 2, when administered in vivo as a vaccine in HLA-A2 transgenic mice, elicit CTLs that lyse target cells expressing the whole protein antigen. Our innovative approach uses dendritic cells transfected with Mtb antigen-encoding mRNA to drive antigen expression. Using this strategy, we have identified five novel peptide epitopes from the Mtb proteins Apa, Mtb8.4 and Mtb19.
Date:2014
Reference Type:Literature
PubMed ID:24755960
Journal:Sci Rep
Journal Volume:4
Article Pages:4632
Journal ISSN:2045-2322
Curation Last Updated:2014-06-09 20:01:09