Epitopes described in "Characterizing monkeypox virus specific CD8+ T cell epitopes in rhesus macaques."

Article Authors:Haifeng Song; John Sidney; Roger W Wiseman; Nicole Josleyn; Melanie Cohen; Joseph E Blaney; Peter B Jahrling; Alessandro Sette
Article Title:Characterizing monkeypox virus specific CD8+ T cell epitopes in rhesus macaques.
Reference Detail
Reference ID:1026998
Abstract:To characterize T cell epitopes in monkeypox virus (MPXV) infected rhesus macaques, we utilized IFN Elispot assay to screen 400 predicted peptides from 20MPXV proteins. Two peptides from the F8L protein, an analog of E9L protein in vaccinia, were found to elicit CD8+ T cell responses. Prediction and in vitro MHC binding analyses suggest that one is restricted by Mamu-A1(⁎)001 and another by Mamu-A1(⁎)002. The Mamu-A1(⁎)002 epitope is completely identical in all reported sequences for variola, vaccinia, cowpox and MPXV. The Mamu-A1(⁎)001 epitope is conserved in MPXV and vaccinia, and has one residue substitution (V6>I) in some cowpox sequences and all variola sequences. Given CD8+ T-cell epitopes from E9L were also identified in humans and mice, our data suggested that F8L/E9L may be a dominant pox viral protein for CD8+ T cell responses, and may be considered as a target when designing vaccines that target pox-specific T cell responses.
Reference Type:Literature
PubMed ID:24210113
Journal Volume:447
Article Pages:181-6
Journal ISSN:0042-6822
Article Chemical List:Epitopes, T-Lymphocyte;Histocompatibility Antigens Class I;Mamu-A 01 antigen;Mamu-A 02 antigen;Interferon-gamma
Article MeSH List:Animals; CD8-Positive T-Lymphocytes(immunology); Enzyme-Linked Immunospot Assay; Epitopes, T-Lymphocyte(immunology); Histocompatibility Antigens Class I(metabolism); Interferon-gamma(secretion); Macaca mulatta; Monkeypox virus(immunology); Protein Binding
Curation Last Updated:2015-06-26 18:12:45