Epitopes described in "Crystal structure of a soluble cleaved HIV-1 envelope trimer."

Article Authors:Jean-Philippe Julien; Albert Cupo; Devin Sok; Robyn L Stanfield; Dmitry Lyumkis; Marc C Deller; Per-Johan Klasse; Dennis R Burton; Rogier W Sanders; John P Moore; Andrew B Ward; Ian A Wilson
Article Title:Crystal structure of a soluble cleaved HIV-1 envelope trimer.
Reference Detail
Reference ID:1026964
Abstract:HIV-1 entry into CD4(+) target cells is mediated by cleaved envelope glycoprotein (Env) trimers that have been challenging to characterize structurally. Here, we describe the crystal structure at 4.7 angstroms of a soluble, cleaved Env trimer that is stabilized and antigenically near-native (termed the BG505 SOSIP.664 gp140 trimer) in complex with a potent broadly neutralizing antibody, PGT122. The structure shows a prefusion state of gp41, the interaction between the component gp120 and gp41 subunits, and how a close association between the gp120 V1/V2/V3 loops stabilizes the trimer apex around the threefold axis. The complete epitope of PGT122 on the trimer involves gp120 V1, V3, and several surrounding glycans. This trimer structure advances our understanding of how Env functions and is presented to the immune system, and provides a blueprint for structure-based vaccine design.
Affiliations:Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Reference Type:Literature
PubMed ID:24179159
Journal Volume:342
Article Pages:1477-83
Journal ISSN:1095-9203
Article Chemical List:gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@3fb08be8;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@5fb14d02;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@7136ef62;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@4d9e44ff;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@51c3e928;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@15006b08;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@4e72e4fd;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@5917c647
Article MeSH List:Antibodies, Neutralizing(chemistry); Antibodies, Viral(chemistry); Crystallography, X-Ray; HIV Envelope Protein gp120(chemistry; immunology); HIV Envelope Protein gp41(chemistry; immunology); Humans; Protein Multimerization; Protein Structure, Quaternary; Recombinant Proteins(chemistry; immunology); Solubility; env Gene Products, Human Immunodeficiency Virus(chemistry; immunology)
Curation Last Updated:2015-01-18 01:10:56