Epitopes described in "Vaccine-induced anti-HA2 antibodies promote virus fusion and enhance influenza virus respiratory disease."

Article Authors:Surender Khurana; Crystal L Loving; Jody Manischewitz; Lisa R King; Phillip C Gauger; Jamie Henningson; Amy L Vincent; Hana Golding
Article Title:Vaccine-induced anti-HA2 antibodies promote virus fusion and enhance influenza virus respiratory disease.
Reference Detail
Reference ID:1026738
Abstract:Vaccine-induced disease enhancement has been described in connection with several viral vaccines in animal models and in humans. We investigated a swine model to evaluate mismatched influenza vaccine-associated enhanced respiratory disease (VAERD) after pH1N1 infection. Vaccinating pigs with whole inactivated H1N2 (human-like) virus vaccine (WIV-H1N2) resulted in enhanced pneumonia and disease after pH1N1 infection. WIV-H1N2 immune sera contained high titers of cross-reactive anti-pH1N1 hemagglutinin (HA) antibodies that bound exclusively to the HA2 domain but not to the HA1 globular head. No hemagglutination inhibition titers against pH1N1 (challenge virus) were measured. Epitope mapping using phage display library identified the immunodominant epitope recognized by WIV-H1N2 immune sera as amino acids 32 to 77 of pH1N1-HA2 domain, close to the fusion peptide. These cross-reactive anti-HA2 antibodies enhanced pH1N1 infection of Madin-Darby canine kidney cells by promoting virus membrane fusion activity. The enhanced fusion activity correlated with lung pathology in pigs. This study suggests a role for fusion-enhancing anti-HA2 antibodies in VAERD, in the absence of receptor-blocking virus-neutralizing antibodies. These findings should be considered during the evaluation of universal influenza vaccines designed to elicit HA2 stem-targeting antibodies.
Reference Type:Literature
PubMed ID:23986398
Journal:Sci Transl Med
Journal Volume:5
Article Pages:200ra114
Journal ISSN:1946-6242
Article Chemical List:Antibodies, Neutralizing;Antibodies, Viral;Epitopes;Hemagglutinin Glycoproteins, Influenza Virus;Immune Sera;Influenza Vaccines
Article MeSH List:Amino Acid Sequence; Animals; Antibodies, Neutralizing(immunology); Antibodies, Viral(immunology); Cross Reactions(immunology); Disease Models, Animal; Dogs; Epitopes(immunology); Hemagglutinin Glycoproteins, Influenza Virus(immunology; metabolism); Humans; Immune Sera(immunology); Influenza A Virus, H1N1 Subtype(immunology); Influenza A Virus, H1N2 Subtype(immunology); Influenza Vaccines(adverse effects; immunology); Influenza, Human(immunology; prevention & control; virology); Madin Darby Canine Kidney Cells; Molecular Sequence Data; Orthomyxoviridae(immunology; pathogenicity); Orthomyxoviridae Infections(immunology; prevention & control; virology); Respiratory Tract Diseases(immunology; prevention & control; virology); Sus scrofa(immunology; virology); Vaccination; Virus Internalization
Curation Last Updated:2014-10-14 18:32:19