Epitopes described in "Characterization of a serologic marker candidate for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus."

Reference
Article Authors:Hiep L X Vu; Byungjoon Kwon; Marcelo de Lima; Asit K Pattnaik; Fernando A Osorio
Article Title:Characterization of a serologic marker candidate for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus.
Reference Detail
Reference ID:1026744
Abstract:DIVA (differentiating infected from vaccinated animals) vaccines have proven extremely useful for control and eradication of infectious diseases in livestock. We describe here the characterization of a serologic marker epitope, so-called epitope-M201, which can be a potential target for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus (PRRSV). Epitope-M201 is located at the carboxyl terminus (residues 161-174) of the viral M protein. The epitope is highly immunodominant and well-conserved among type-II PRRSV isolates. Rabbit polyclonal antibodies prepared against this epitope are non-neutralizing; thus, the epitope does not seem to contribute to the protective immunity against PRRSV infection. Importantly, the immunogenicity of epitope-M201 can be disrupted through the introduction of a single amino acid mutation which does not adversely affect the viral replication. All together, our results provide an important starting point for the development of a live-attenuated DIVA vaccine against type-II PRRSV.
Date:2013
Reference Type:Literature
PubMed ID:23892102
Journal:Vaccine
Journal Volume:31
Article Pages:4330-7
Journal ISSN:0264-410X
Article Chemical List:Antibodies, Viral;Epitopes;Glycoproteins;Vaccines, Attenuated;Viral Vaccines;protein M (glycoprotein)
Article MeSH List:Amino Acid Sequence; Animals; Antibodies, Viral(immunology); Epitopes(blood; immunology); Glycoproteins(immunology); Mice; Porcine Reproductive and Respiratory Syndrome(immunology; prevention & control); Porcine respiratory and reproductive syndrome virus(genetics; immunology); Swine; Swine Diseases(immunology; prevention & control); Vaccination(veterinary); Vaccines, Attenuated(immunology); Viral Vaccines(genetics; immunology)
Curation Last Updated:2014-07-16 23:19:04