Epitopes described in "Protective CD8 T cell-mediated immunity against influenza A virus infection following influenza virus-like particle vaccination."

Article Authors:Emily A Hemann; Sang-Moo Kang; Kevin L Legge
Article Title:Protective CD8 T cell-mediated immunity against influenza A virus infection following influenza virus-like particle vaccination.
Reference Detail
Reference ID:1026733
Abstract:The development of influenza A virus (IAV) vaccines capable of inducing cytotoxic CD8 T cell responses could potentially provide superior, long-term protection against multiple, heterologous strains of IAV. Although prior studies demonstrated the effectiveness of baculovirus-derived virus-like particle (VLP) vaccination in generating Ab-mediated protection, the role that CD8 T cell immunity plays in overall VLP-mediated protection is less-well understood. In this article, we demonstrate that intranasal vaccination of mice with a VLP containing the hemagglutinin and matrix 1 proteins of IAV/PR/8/34 leads to a significant increase in hemagglutinin 533-specific CD8 T cells in the lungs and protection following subsequent homologous challenge with IAV. VLP-mediated protection was significantly reduced by CD8 T cell depletion, indicating a critical role for CD8 T cells in protective immunity. Importantly, our results show that VLP vaccine-induced CD8 T cell-mediated protection is not limited to homologous IAV strains. VLP vaccination leads to an increase in protection following heterosubtypic challenge with a strain of IAV that avoids vaccine-induced neutralizing Abs but contains conserved, immunodominant CD8 T cell epitopes. Overall, our results demonstrate the ability of influenza protein-containing VLPs to prime IAV-specific CD8 T cell responses that contribute to protection from homo- and heterosubtypic IAV infections. These results further suggest that vaccination strategies focused on the development of cross-protective CD8 T cell responses may contribute to the development of "universal" IAV vaccines.
Affiliations:Department of Pathology and Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242, USA.
Reference Type:Literature
PubMed ID:23885108
Journal:J Immunol
Journal Volume:191
Article Pages:2486-94
Journal ISSN:0022-1767
Article Chemical List:Influenza Vaccines;Vaccines, Virus-Like Particle;Viral Matrix Proteins
Article MeSH List:Animals; CD8-Positive T-Lymphocytes(immunology); Influenza A virus(immunology); Influenza Vaccines(immunology); Mice; Mice, Inbred BALB C; Orthomyxoviridae Infections(immunology; prevention & control); Vaccines, Virus-Like Particle(immunology); Viral Matrix Proteins(immunology)
Curation Last Updated:2015-07-30 20:48:37