Epitopes described in "Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid."

Article Authors:Gabriel I Parra; Jolynn Azure; Renate Fischer; Karin Bok; Carlos Sandoval-Jaime; Stanislav V Sosnovtsev; Peter Sander; Kim Y Green
Article Title:Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid.
Reference Detail
Reference ID:1026460
Abstract:Noroviruses are major pathogens associated with acute gastroenteritis. They are diverse viruses, with at least six genogroups (GI-GVI) and multiple genotypes defined by differences in the major capsid protein, VP1. This diversity has challenged the development of broadly cross-reactive vaccines as well as efficient detection methods. Here, we report the characterization of a broadly cross-reactive monoclonal antibody (MAb) raised against the capsid protein of a GII.3 norovirus strain. The MAb reacted with VLPs and denatured VP1 protein from GI, GII, GIV and GV noroviruses, and mapped to a linear epitope located in the inner shell domain. An alignment of all available VP1 sequences showed that the putative epitope (residues 52-56) is highly conserved across the genus Norovirus. This broadly cross-reactive MAb thus constitutes a valuable reagent for the diagnosis and study of these diverse viruses.
Affiliations:Caliciviruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, DHHS, Bethesda, Maryland, United States of America.
Reference Type:Literature
PubMed ID:23805319
Journal:PLoS ONE
Journal Volume:8
Article Pages:e67592
Journal ISSN:1932-6203
Curation Last Updated:2014-10-03 22:56:32