Epitopes described in "Delineating antibody recognition in polyclonal sera from patterns of HIV-1 isolate neutralization."

Article Authors:Ivelin S Georgiev; Nicole A Doria-Rose; Tongqing Zhou; Young Do Kwon; Ryan P Staupe; Stephanie Moquin; Gwo-Yu Chuang; Mark K Louder; Stephen D Schmidt; Han R Altae-Tran; Robert T Bailer; Krisha McKee; Martha Nason; Sijy O'Dell; Gilad Ofek; Marie Pancera; Sanjay Srivatsan; Lawrence Shapiro; Mark Connors; Stephen A Migueles; Lynn Morris; Yoshiaki Nishimura; Malcolm A Martin; John R Mascola; Peter D Kwong
Article Title:Delineating antibody recognition in polyclonal sera from patterns of HIV-1 isolate neutralization.
Reference Detail
Reference ID:1025842
Abstract:Serum characterization and antibody isolation are transforming our understanding of the humoral immune response to viral infection. Here, we show that epitope specificities of HIV-1-neutralizing antibodies in serum can be elucidated from the serum pattern of neutralization against a diverse panel of HIV-1 isolates. We determined "neutralization fingerprints" for 30 neutralizing antibodies on a panel of 34 diverse HIV-1 strains and showed that similarity in neutralization fingerprint correlated with similarity in epitope. We used these fingerprints to delineate specificities of polyclonal sera from 24 HIV-1-infected donors and a chimeric siman-human immunodeficiency virus-infected macaque. Delineated specificities matched published specificities and were further confirmed by antibody isolation for two sera. Patterns of virus-isolate neutralization can thus afford a detailed epitope-specific understanding of neutralizing-antibody responses to viral infection.
Affiliations:Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Reference Type:Literature
PubMed ID:23661761
Journal Volume:340
Article Pages:751-6
Journal ISSN:1095-9203
Article Chemical List:Antibodies, Neutralizing;HIV Antibodies;Immunodominant Epitopes
Article MeSH List:Animals; Antibodies, Neutralizing(blood; immunology); Epitope Mapping; HIV Antibodies(blood; immunology); HIV Infections(blood; immunology); HIV-1(immunology; isolation & purification); Humans; Immunodominant Epitopes(chemistry; immunology); Macaca; Neutralization Tests; Protein Conformation; Serum(immunology)
Curation Last Updated:2016-05-11 20:11:50