Epitopes described in "Examination of influenza specific T cell responses after influenza virus challenge in individuals vaccinated with MVA-NP+M1 vaccine."

Article Authors:Timothy J Powell; Yanchun Peng; Tamara K Berthoud; Marie-Eve Blais; Patrick J Lillie; Adrian V S Hill; Sarah L Rowland-Jones; Andrew J McMichael; Sarah C Gilbert; Tao Dong
Article Title:Examination of influenza specific T cell responses after influenza virus challenge in individuals vaccinated with MVA-NP+M1 vaccine.
Reference Detail
Reference ID:1025797
Abstract:Current influenza vaccines stimulate neutralising antibody to the haemagglutinin antigen but as there is antigenic drift in HA it is difficult to prepare a vaccine in advance against an emergent strain. A potential strategy is to induce CD8(+) and CD4(+) T cells that recognize epitopes within internal proteins that are less subject to antigenic drift. Augmenting humoral responses to HA with T cell responses to more conserved antigens may result in a more broadly protective vaccine. In this study, we evaluate the quality of influenza specific T cell responses in a clinical trial using MVA-NP+M1 vaccination followed by influenza virus challenge. In vaccinated volunteers, the expression of Granzyme A, Perforin and CD57 on influenza HLA A*02 M158-66 antigen specific cells was higher than non-vaccinated volunteers before and after challenge despite a similar frequency of antigen specific cells. BCL2 expression was lower in vaccinated volunteers. These data indicate that antigen specific T cells are a useful additional measure for use in human vaccination or immunization studies.
Affiliations:MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom. timothy.powell@imm.ox.ac.uk.
Reference Type:Literature
PubMed ID:23658773
Journal:PLoS One
Journal Volume:8
Article Pages:e62778
Journal ISSN:1932-6203
Article Chemical List:Antigens, CD57;Antigens, Viral;Epitopes, T-Lymphocyte;HLA-A2 Antigen;Hemagglutinins;Influenza Vaccines;M1 protein, Influenza A virus;MVA vaccine;Nucleoproteins;Recombinant Proteins;Vaccines, Synthetic;Viral Core Proteins;Viral Matrix Proteins;Viral Vaccines;Perforin;Granzymes
Article MeSH List:Adolescent; Adult; Antigens, CD57(genetics; immunology); Antigens, Viral(genetics; immunology); CD8-Positive T-Lymphocytes(immunology; virology); Epitopes, T-Lymphocyte(genetics; immunology); Granzymes(genetics; immunology); HLA-A2 Antigen(genetics; immunology); Hemagglutinins(genetics; immunology); Humans; Influenza Vaccines(administration & dosage; genetics; immunology); Influenza, Human(genetics; immunology; prevention & control); Middle Aged; Nucleoproteins(genetics; immunology); Orthomyxoviridae(drug effects; immunology); Perforin(genetics; immunology); Recombinant Proteins(genetics; immunology); Vaccination; Vaccines, Synthetic; Viral Core Proteins(genetics; immunology); Viral Matrix Proteins(genetics; immunology); Viral Vaccines(administration & dosage; genetics; immunology)
Curation Last Updated:2015-06-05 03:58:01