Epitopes described in "Human endogenous retrovirus K(HML-2) Gag and Env specific T-cell responses are not detected in HTLV-I-infected subjects using standard peptide screening methods."

Article Authors:R Brad Jones; Fabio E Leal; Aaron M Hasenkrug; Aluisio C Segurado; Douglas F Nixon; Mario A Ostrowski; Esper G Kallas
Article Title:Human endogenous retrovirus K(HML-2) Gag and Env specific T-cell responses are not detected in HTLV-I-infected subjects using standard peptide screening methods.
Reference Detail
Reference ID:1025488
Abstract:BACKGROUND: An estimated 10-20 million individuals are infected with the retrovirus human T-cell leukemia virus type 1 (HTLV-1). While the majority of these individuals remain asymptomatic, 0.3-4% develop a neurodegenerative inflammatory disease, termed HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HAM/TSP results in the progressive demyelination of the central nervous system and is a differential diagnosis of multiple sclerosis (MS). The etiology of HAM/TSP is unclear, but evidence points to a role for CNS-inflitrating T-cells in pathogenesis. Recently, the HTLV-1-Tax protein has been shown to induce transcription of the human endogenous retrovirus (HERV) families W, H and K. Intriguingly, numerous studies have implicated these same HERV families in MS, though this association remains controversial. RESULTS: Here, we explore the hypothesis that HTLV-1-infection results in the induction of HERV antigen expression and the elicitation of HERV-specific T-cells responses which, in turn, may be reactive against neurons and other tissues. PBMC from 15 HTLV-1-infected subjects, 5 of whom presented with HAM/TSP, were comprehensively screened for T-cell responses to overlapping peptides spanning HERV-K(HML-2) Gag and Env. In addition, we screened for responses to peptides derived from diverse HERV families, selected based on predicted binding to predicted optimal epitopes. We observed a lack of responses to each of these peptide sets. CONCLUSIONS: Thus, although the limited scope of our screening prevents us from conclusively disproving our hypothesis, the current study does not provide data supporting a role for HERV-specific T-cell responses in HTLV-1 associated immunopathology.
Affiliations:Department of Immunology, University of Toronto, 1 King's College Circle, Rm 6352, Toronto, ON M5S 1A8, Canada. brad.jones@utoronto.ca.
Reference Type:Literature
PubMed ID:23305161
Journal:J Negat Results Biomed
Journal Volume:12
Article Pages:3
Journal ISSN:1477-5751
Article Chemical List:gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@4d36f6dd;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@53894ec5
Article MeSH List:Adult; Endogenous Retroviruses(immunology); Female; Gene Products, env(immunology); Gene Products, gag(immunology); HTLV-I Infections(immunology); Humans; Male; Middle Aged; T-Lymphocytes(immunology); Young Adult
Curation Last Updated:2015-04-29 21:42:24