Epitopes described in "CSP--a model for in vivo presentation of Plasmodium berghei sporozoite antigens by hepatocytes."

Article Authors:Saidou Balam; Jackeline F Romero; Silayuv E Bongfen; Philippe Guillaume; Giampietro Corradin
Article Title:CSP--a model for in vivo presentation of Plasmodium berghei sporozoite antigens by hepatocytes.
Reference Detail
Reference ID:1025407
Abstract:One target of protective immunity against the Plasmodium liver stage in BALB/c mice is represented by the circumsporozoite protein (CSP), and mainly involves its recognition by IFN- producing specific CD8+T-cells. In a previous in vitro study we showed that primary hepatocytes from BALB/c mice process Plasmodium berghei (Pb) CSP (PbCSP) and present CSP-derived peptides to specific H-2k(d) restricted CD8+T-cells with subsequent killing of the presenting cells. We now extend these observations to an in vivo infection model in which infected hepatocytes and antigen specific T-cell clones are transferred into recipient mice inducing protection from sporozoite (SPZ) challenge. In addition, using a similar protocol, we suggest the capacity of hepatocytes in priming of naïve T-cells to provide protection, as further confirmed by induction of protection after depletion of cross-presenting dendritic cells (DCs) by cytochrome c (cyt c) treatment or using traversal deficient parasites. Our results clearly show that hepatocytes present Plasmodium CSP to specific-primed CD8+T-cells, and could also prime naïve T-cells, leading to protection from infection. These results could contribute to a better understanding of liver stage immune response and design of malaria vaccines.
Affiliations:Department of Biochemistry, University of Lausanne, Epalinges, Switzerland.
Reference Type:Literature
PubMed ID:23272182
Journal:PLoS One
Journal Volume:7
Article Pages:e51875
Journal ISSN:1932-6203
Article Chemical List:Antigens, Protozoan;Cytokines;Epitopes, T-Lymphocyte;Protozoan Proteins;circumsporozoite protein, Protozoan;Cytochromes c
Article MeSH List:Animals; Antigen Presentation; Antigens, Protozoan(immunology); CD8-Positive T-Lymphocytes(immunology); Cytochromes c(administration & dosage); Cytokines(metabolism); Epitopes, T-Lymphocyte(immunology); Hepatocytes(immunology; parasitology); Lymphocyte Activation(immunology); Malaria(immunology; parasitology); Mice; Plasmodium berghei(immunology); Protozoan Proteins(immunology); Spleen(immunology; parasitology); Sporozoites(immunology)
Curation Last Updated:2015-07-30 20:46:50