Epitopes described in "CD4 T-cell memory responses to viral infections of humans show pronounced immunodominance independent of duration or viral persistence."

Article Authors:Lichen Jing; Joshua T Schiffer; Tiana M Chong; Joseph J Bruckner; D Huw Davies; Phillip L Felgner; Juergen Haas; Anna Wald; G M G M Verjans; David M Koelle
Article Title:CD4 T-cell memory responses to viral infections of humans show pronounced immunodominance independent of duration or viral persistence.
Reference Detail
Reference ID:1025542
Abstract:Little is known concerning immunodominance within the CD4 T-cell response to viral infections and its persistence into long-term memory. We tested CD4 T-cell reactivity against each viral protein in persons immunized with vaccinia virus (VV), either recently or more than 40 years ago, as a model self-limited viral infection. Similar tests were done with persons with herpes simplex virus 1 (HSV-1) infection as a model chronic infection. We used an indirect method capable of counting the CD4 T cells in blood reactive with each individual viral protein. Each person had a clear CD4 T-cell dominance hierarchy. The top four open reading frames accounted for about 40% of CD4 virus-specific T cells. Early and long-term memory CD4 T-cell responses to vaccinia virus were mathematically indistinguishable for antigen breadth and immunodominance. Despite the chronic intermittent presence of HSV-1 antigen, the CD4 T-cell dominance and diversity patterns for HSV-1 were identical to those observed for vaccinia virus. The immunodominant CD4 T-cell antigens included both long proteins abundantly present in virions and shorter, nonstructural proteins. Limited epitope level and direct ex vivo data were also consistent with pronounced CD4 T-cell immunodominance. We conclude that human memory CD4 T-cell responses show a pattern of pronounced immunodominance for both chronic and self-limited viral infections and that this pattern can persist over several decades in the absence of antigen.
Affiliations:Department of Medicine, University of Washington, Seattle, Washington, USA.
Reference Type:Literature
PubMed ID:23255792
Journal:J Virol
Journal Volume:87
Article Pages:2617-27
Journal ISSN:1098-5514
Article Chemical List:gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@18fb2422;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@100140fe;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@4251ebe7;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@1056acb5;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@2a64a774;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@362dc66a;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@773d74ef
Article MeSH List:Antigens, CD137(biosynthesis); CD4-Positive T-Lymphocytes(immunology); CD8-Positive T-Lymphocytes(immunology); Cells, Cultured; Herpes Simplex(immunology); Herpesvirus 1, Human(immunology); Humans; Immunity, Cellular; Immunologic Memory; Interferon-gamma(biosynthesis); Interleukin-2(biosynthesis); Smallpox Vaccine(immunology); Tumor Necrosis Factor-alpha(biosynthesis); Vaccines, Attenuated; Vaccinia virus(immunology)
Curation Last Updated:2015-01-18 00:42:32