Epitopes described in "Maternally transmitted histocompatibility antigen of mice: a hydrophobic peptide of a mitochondrially encoded protein."

Article Authors:B Loveland; C R Wang; H Yonekawa; E Hermel; K F Lindahl
Article Title:Maternally transmitted histocompatibility antigen of mice: a hydrophobic peptide of a mitochondrially encoded protein.
Reference Detail
Reference ID:1020326
Abstract:MTF, a murine minor histocompatibility antigen, is maternally inherited and thought to be encoded by a mitochondrial gene. We sequenced the entire mitochondrial genomes from three strains that differ in MTF Mtf beta, Mtf gamma, and Mtf delta) and compared the sequences with the known, Mtf alpha, mitochondrial DNA sequence. We found only one site where all four genomes differed, affecting amino acid residue 6 of ND1, a subunit of NADH dehydrogenase. Incubation of non-Mtf alpha target cells with synthetic peptide ND1 alpha 1-17 (the first 17 amino acid of the ND1 protein of Mtf alpha mice) rendered them susceptible to lysis by MTF alpha-specific cytotoxic T cells (CTLs). Similarly, non-Mtf beta target cells were lysed by MTF beta-specific CTLs after incubation with the allelic form ND1 beta 1-17. Thus, Mtf is attributable to allelic variation at a single residue of the ND1 protein. Cells can therefore display peptides derived from mitochondrially encoded proteins, and such peptides can be histocompatibility antigens.
Affiliations:Howard Hughes Medical Institute, Department of Microbiology, Dallas, Texas.
Reference Type:Literature
PubMed ID:2317868
Journal Volume:60
Article Pages:971-80
Journal ISSN:0092-8674
Article Chemical List:DNA, Mitochondrial;Minor Histocompatibility Antigens;Peptides
Article MeSH List:Amino Acid Sequence; Animals; Cell Line; Cloning, Molecular; Cytotoxicity, Immunologic; DNA, Mitochondrial(genetics); Mice; Minor Histocompatibility Antigens(genetics); Minor Histocompatibility Loci; Mitochondria(immunology); Molecular Sequence Data; Peptides(chemical synthesis); Polymorphism, Genetic; T-Lymphocytes, Cytotoxic(immunology)
Curation Last Updated:2015-07-30 20:37:13