Epitopes described in "MHC class I-presented T cell epitopes identified by immunoproteomics analysis are targets for a cross reactive influenza-specific T cell response."

Article Authors:James S Testa; Vivekananda Shetty; Julie Hafner; Zacharie Nickens; Shivali Kamal; Gomathinayagam Sinnathamby; Ramila Philip
Article Title:MHC class I-presented T cell epitopes identified by immunoproteomics analysis are targets for a cross reactive influenza-specific T cell response.
Reference Detail
Reference ID:1025287
Abstract:Influenza virus infection and the resulting complications are a significant global public health problem. Improving humoral immunity to influenza is the target of current conventional influenza vaccines, however, these are generally not cross-protective. On the contrary, cell-mediated immunity generated by primary influenza infection provides substantial protection against serologically distinct viruses due to recognition of cross-reactive T cell epitopes, often from internal viral proteins conserved between viral subtypes. Efforts are underway to develop a universal flu vaccine that would stimulate both the humoral and cellular immune responses leading to long-lived memory. Such a universal vaccine should target conserved influenza virus antibody and T cell epitopes that do not vary from strain to strain. In the last decade, immunoproteomics, or the direct identification of HLA class I presented epitopes, has emerged as an alternative to the motif prediction method for the identification of T cell epitopes. In this study, we used this method to uncover several cross-specific MHC class I specific T cell epitopes naturally presented by influenza A-infected cells. These conserved T cell epitopes, when combined with a cross-reactive antibody epitope from the ectodomain of influenza M2, generate cross-strain specific cell mediated and humoral immunity. Overall, we have demonstrated that conserved epitope-specific CTLs could recognize multiple influenza strain infected target cells and, when combined with a universal antibody epitope, could generate virus specific humoral and T cell responses, a step toward a universal vaccine concept. These epitopes also have potential as new tools to characterize T cell immunity in influenza infection, and may serve as part of a universal vaccine candidate complementary to current vaccines.
Affiliations:Immunotope, Inc., Doylestown, PA, USA.
Reference Type:Literature
PubMed ID:23144892
Journal:PLoS One
Journal Volume:7
Article Pages:e48484
Journal ISSN:1932-6203
Article Chemical List:Antibodies, Neutralizing;Antibodies, Viral;Epitopes, T-Lymphocyte;Histocompatibility Antigens Class I;Peptides
Article MeSH List:Animals; Antibodies, Neutralizing(immunology); Antibodies, Viral(immunology); Antigen Presentation(immunology); Chromatography, Liquid; Cross Reactions(immunology); Epitopes, T-Lymphocyte(immunology); Female; Hep G2 Cells; Histocompatibility Antigens Class I(chemistry; immunology); Humans; Influenza A virus(immunology); Influenza, Human(immunology; virology); Mass Spectrometry; Mice; Mice, Transgenic; Orthomyxoviridae Infections(immunology; virology); Peptides(chemistry; immunology); Proteomics(methods); Reproducibility of Results; T-Lymphocytes, Cytotoxic(immunology)
Curation Last Updated:2015-06-05 03:45:05