Epitopes described in "Pre-existing virus-specific CD8(+) T-cells provide protection against pneumovirus-induced disease in mice."

Article Authors:Mary J G van Helden; Peter J S van Kooten; Cornelis P J Bekker; Andrea Gröne; David J Topham; Andrew J Easton; Claire J P Boog; Dirk H Busch; Dietmar M W Zaiss; Alice J A M Sijts
Article Title:Pre-existing virus-specific CD8(+) T-cells provide protection against pneumovirus-induced disease in mice.
Reference Detail
Reference ID:1025134
Abstract:Pneumoviruses such as pneumonia virus of mice (PVM), bovine respiratory syncytial virus (bRSV) or human (h)RSV are closely related pneumoviruses that cause severe respiratory disease in their respective hosts. It is well-known that T-cell responses are essential in pneumovirus clearance, but pneumovirus-specific T-cell responses also are important mediators of severe immunopathology. In this study we determined whether memory- or pre-existing, transferred virus-specific CD8(+) T-cells provide protection against PVM-induced disease. We show that during infection with a sublethal dose of PVM, both natural killer (NK) cells and CD8(+) T-cells expand relatively late. Induction of CD8(+) T-cell memory against a single CD8(+) T-cell epitope, by dendritic cell (DC)-peptide immunization, leads to partial protection against PVM challenge and prevents Th2 differentiation of PVM-induced CD4 T-cells. In addition, adoptively transferred PVM-specific CD8(+) T-cells, covering the entire PVM-specific CD8(+) T-cell repertoire, provide partial protection from PVM-induced disease. From these data we infer that antigen-specific memory CD8(+) T-cells offer significant protection to PVM-induced disease. Thus, CD8(+) T-cells, despite being a major cause of PVM-associated pathology during primary infection, may offer promising targets of a protective pneumovirus vaccine.
Affiliations:Division of Immunology, University of Utrecht, Yalelaan 1, 3584 CL Utrecht, The Netherlands.
Reference Type:Literature
PubMed ID:22940382
Journal Volume:30
Article Pages:6382-8
Journal ISSN:0264-410X
Article Chemical List:Interleukin-4;Interferon-gamma
Article MeSH List:Adoptive Transfer; Animals; CD8-Positive T-Lymphocytes(immunology); Female; Immunologic Memory; Influenza A Virus, H3N2 Subtype(immunology); Interferon-gamma(immunology); Interleukin-4(immunology); Killer Cells, Natural(immunology); Mice; Mice, Inbred BALB C; Murine pneumonia virus(immunology); Pneumovirus Infections(immunology); Respiratory Syncytial Viruses(immunology)
Curation Last Updated:2015-07-30 20:46:24