Epitopes described in "Effect of vaccine dose on the safety and immunogenicity of a candidate TB vaccine, MVA85A, in BCG vaccinated UK adults."

Article Authors:Ansar A Pathan; Angela M Minassian; Clare R Sander; Rosalind Rowland; David W Porter; Ian D Poulton; Adrian V S Hill; Helen A Fletcher; Helen McShane
Article Title:Effect of vaccine dose on the safety and immunogenicity of a candidate TB vaccine, MVA85A, in BCG vaccinated UK adults.
Reference Detail
Reference ID:1024984
Abstract:PURPOSE: A non-randomised, open-label, Phase I safety and immunogenicity dose-finding study to assess the safety and immunogenicity of the candidate TB vaccine Modified Vaccinia virus Ankara expressing Antigen 85A (MVA85A) from Mycobacterium tuberculosis (MTB) in healthy adult volunteers previously vaccinated with BCG. METHODS: Healthy BCG-vaccinated volunteers were vaccinated with either 1×10(7) or 1×10(8)PFU of MVA85A. All adverse events were documented and antigen specific T cell responses were measured using an ex vivo IFN- ELISPOT assay. Safety and immunogenicity were compared between the 2 dose groups and with a previous trial in which a dose of 5×10(7)PFU MVA85A had been administered. RESULTS: There were no serious adverse events recorded following administration of either 1×10(7) or 1×10(8)PFU of MVA85A. Systemic adverse events were more frequently reported following administration of 1×10(8)PFU of MVA85A when compared to either 5×10(7) or 1×10(7)PFU of MVA85A but were mild or moderate in severity and resolved completely within 7 days of immunisation. Antigen specific T cell responses as measured by the IFN- ELISPOT were significantly higher following immunisation in adults receiving 1×10(8)PFU compared to the 5×10(7) and 1×10(7) doses. Additionally, a broader range of Ag85A epitopes are detected following 1×10(8)PFU of MVA85A. CONCLUSION: A higher dose of 1×10(8)PFU of MVA85A is well-tolerated, increases the frequency of IFN- secreting T cells detected following immunisation and broadens the range of Ag85A epitopes detected.
Affiliations:The Jenner Institute, University of Oxford, Oxford, United Kingdom.
Reference Type:Literature
PubMed ID:22789508
Journal Volume:30
Article Pages:5616-24
Journal ISSN:0264-410X
Article Chemical List:gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@2594358e;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@254810e8;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@1e4e0277;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@1383e22c
Article MeSH List:Adult; Drug-Related Side Effects and Adverse Reactions(epidemiology); Enzyme-Linked Immunospot Assay; Female; Great Britain; Human Experimentation; Humans; Interferon-gamma(secretion); Male; Middle Aged; T-Lymphocytes(immunology); Tuberculosis(prevention & control); Tuberculosis Vaccines(administration & dosage; adverse effects; immunology); Viral Vaccines(administration & dosage; adverse effects; immunology); Young Adult
Curation Last Updated:2015-01-18 20:18:00