Epitopes described in "A human CD4+ T cell epitope in the influenza hemagglutinin is cross-reactive to influenza A virus subtypes and to influenza B virus."

Article Authors:Jenny Aurielle B Babon; John Cruz; Francis A Ennis; Liusong Yin; Masanori Terajima
Article Title:A human CD4+ T cell epitope in the influenza hemagglutinin is cross-reactive to influenza A virus subtypes and to influenza B virus.
Reference Detail
Reference ID:1024990
Abstract:The hemagglutinin protein (HA) of the influenza virus family is a major antigen for protective immunity. Thus, it is a relevant target for developing vaccines. Here, we describe a human CD4(+) T cell epitope in the influenza virus HA that lies in the fusion peptide of the HA. This epitope is well conserved in all 16 subtypes of the HA protein of influenza A virus and the HA protein of influenza B virus. By stimulating peripheral blood mononuclear cells (PBMCs) from a healthy adult donor with peptides covering the entire HA protein based on the sequence of A/Japan/305/1957 (H2N2), we generated a T cell line specific to this epitope. This CD4(+) T cell line recognizes target cells infected with influenza A virus seasonal H1N1 and H3N2 strains, a reassortant H2N1 strain, the 2009 pandemic H1N1 strain, and influenza B virus in cytotoxicity assays and intracellular-cytokine-staining assays. It also lysed target cells infected with avian H5N1 virus. We screened healthy adult PBMCs for T cell responses specific to this epitope and found individuals who had ex vivo gamma interferon (IFN-) responses to the peptide epitope in enzyme-linked immunospot (ELISPOT) assays. Almost all donors who responded to the epitope had the HLA-DRB1*09 allele, a relatively common HLA allele. Although natural infection or standard vaccination may not induce strong T and B cell responses to this highly conserved epitope in the fusion peptide, it may be possible to develop a vaccination strategy to induce these CD4(+) T cells, which are cross-reactive to both influenza A and B viruses.
Affiliations:Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Reference Type:Literature
PubMed ID:22718815
Journal:J Virol
Journal Volume:86
Article Pages:9233-43
Journal ISSN:1098-5514
Article Chemical List:Antibodies, Viral;Epitopes, T-Lymphocyte;Hemagglutinin Glycoproteins, Influenza Virus;Histocompatibility Antigens Class I
Article MeSH List:Antibodies, Viral(immunology); CD4-Positive T-Lymphocytes(immunology); Cells, Cultured; Cross Reactions; Epitopes, T-Lymphocyte(genetics; immunology); Hemagglutinin Glycoproteins, Influenza Virus(genetics; immunology); Histocompatibility Antigens Class I(genetics; immunology); Humans; Influenza A Virus, H1N1 Subtype(genetics; immunology); Influenza A Virus, H2N2 Subtype(genetics; immunology); Influenza A Virus, H3N2 Subtype(genetics; immunology); Influenza A virus(genetics; immunology); Influenza B virus(genetics; immunology); Influenza, Human(genetics; immunology; virology); Leukocytes, Mononuclear(immunology)
Article Comments:ErratumIn(J Virol. 2013 Aug;87(16):9396 )
Curation Last Updated:2016-01-08 22:10:13