Epitopes described in "Anti-HPV16 E2 protein T-cell responses and viral control in women with usual vulvar intraepithelial neoplasia and their healthy partners."

Article Authors:Simon Jacobelli; Fedoua Sanaa; Micheline Moyal-Barracco; Monique Pelisse; Sophie Berville; Pascale Villefroy; Marie Odile North; Suzanne Figueiredo; Bénédicte Charmeteau; Thierry Clerici; Françoise Plantier; Françoise Arnold; Antoine Touzé; Nicolas Dupin; Marie-Françoise Avril; Jean-Gérard Guillet; Rémi Cheynier; Isabelle Bourgault-Villada
Article Title:Anti-HPV16 E2 protein T-cell responses and viral control in women with usual vulvar intraepithelial neoplasia and their healthy partners.
Reference Detail
Reference ID:1024191
Abstract:T-cell responses (proliferation, intracellular cytokine synthesis and IFN ELISPOT) against human papillomavirus 16 (HPV16) E2 peptides were tested during 18 months in a longitudinal study in eight women presenting with HPV16-related usual vulvar intraepithelial neoplasia (VIN) and their healthy male partners. In six women, anti-E2 proliferative responses and cytokine production (single IFN and/or dual IFN/IL2 and/or single IL2) by CD4+ T lymphocytes became detectable after treating and healing of the usual VIN. In the women presenting with persistent lesions despite therapy, no proliferation was observed. Anti-E2 proliferative responses were also observed with dual IFN/IL2 production by CD4+ T-cells in six male partners who did not exhibit any genital HPV-related diseases. Ex vivo IFN ELISPOT showed numerous effector T-cells producing IFN after stimulation by a dominant E2 peptide in all men and women. Since the E2 protein is absent from the viral particles but is required for viral DNA replication, these results suggest a recent infection with replicative HPV16 in male partners. The presence of polyfunctional anti-E2 T-cell responses in the blood of asymptomatic men unambiguously establishes HPV infection even without detectable lesions. These results, despite the small size of the studied group, provide an argument in favor of prophylactic HPV vaccination of young men in order to prevent HPV16 infection and viral transmission from men to women.
Affiliations:Institut Cochin, Université Paris Descartes, CNRS, UMR 8104, Paris, France. simonjacobelli@gmail.com.
Reference Type:Literature
PubMed ID:22590583
Journal:PLoS One
Journal Volume:7
Article Pages:e36651
Journal ISSN:1932-6203
Article Chemical List:DNA, Viral;DNA-Binding Proteins;E2 protein, Human papillomavirus type 16;IL2 protein, human;Interleukin-2;Oncogene Proteins, Viral;Interferon-gamma
Article MeSH List:Adult; CD4-Positive T-Lymphocytes(immunology; pathology); DNA, Viral(immunology); DNA-Binding Proteins(immunology); Enzyme-Linked Immunospot Assay; Female; Human papillomavirus 16(physiology); Humans; Immunity, Cellular; Interferon-gamma(immunology); Interleukin-2(immunology); Male; Middle Aged; Oncogene Proteins, Viral(immunology); Papillomavirus Infections(immunology; pathology; transmission); Virus Replication(immunology); Vulvar Neoplasms(immunology; pathology; veterinary)
Curation Last Updated:2015-06-07 20:14:43