Epitopes described in "Pathogen-specific epitopes as epidemiological tools for defining the magnitude of Mycobacterium leprae transmission in areas endemic for leprosy."

Article Authors:Marcia V S B Martins; Marjorie M da S Guimarães; John S Spencer; Mariana A V B Hacker; Luciana S Costa; Fernanda M Carvalho; Annemieke Geluk; Jolien J van der Ploeg-van Schip; Maria A A Pontes; Heitor S Gonçalves; Janvier P de Morais; Tereza J P G Bandeira; Maria C V Pessolani; Patrick J Brennan; Geraldo M B Pereira
Article Title:Pathogen-specific epitopes as epidemiological tools for defining the magnitude of Mycobacterium leprae transmission in areas endemic for leprosy.
Reference Detail
Reference ID:1023095
Abstract:During recent years, comparative genomic analysis has allowed the identification of Mycobacterium leprae-specific genes with potential application for the diagnosis of leprosy. In a previous study, 58 synthetic peptides derived from these sequences were tested for their ability to induce production of IFN- in PBMC from endemic controls (EC) with unknown exposure to M. leprae, household contacts of leprosy patients and patients, indicating the potential of these synthetic peptides for the diagnosis of sub- or preclinical forms of leprosy. In the present study, the patterns of IFN- release of the individuals exposed or non-exposed to M. leprae were compared using an Artificial Neural Network algorithm, and the most promising M. leprae peptides for the identification of exposed people were selected. This subset of M. leprae-specific peptides allowed the differentiation of groups of individuals from sites hyperendemic for leprosy versus those from areas with lower level detection rates. A progressive reduction in the IFN- levels in response to the peptides was seen when contacts of multibacillary (MB) patients were compared to other less exposed groups, suggesting a down modulation of IFN- production with an increase in bacillary load or exposure to M. leprae. The data generated indicate that an IFN- assay based on these peptides applied individually or as a pool can be used as a new tool for predicting the magnitude of M. leprae transmission in a given population.
Affiliations:Laboratory of Cellular Microbiology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
Reference Type:Literature
PubMed ID:22545169
Journal:PLoS Negl Trop Dis
Journal Volume:6
Article Pages:e1616
Journal ISSN:1935-2735
Article Chemical List:gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@274e57e8;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@86d5481
Article MeSH List:Adult; Aged; Antigens, Bacterial(diagnostic use; immunology); Epitopes(immunology); Female; Humans; Interferon-gamma Release Tests(methods); Leprosy(diagnosis; transmission); Male; Middle Aged; Mycobacterium leprae(immunology); Neural Networks (Computer)
Curation Last Updated:2015-01-18 00:19:34