Epitopes described in "Strong immunogenicity and cross-reactivity of Mycobacterium tuberculosis ESX-5 type VII secretion: encoded PE-PPE proteins predicts vaccine potential."

Reference
Article Authors:Fadel Sayes; Lin Sun; Mariagrazia Di Luca; Roxane Simeone; Nathalie Degaiffier; Laurence Fiette; Semih Esin; Roland Brosch; Daria Bottai; Claude Leclerc; Laleh Majlessi
Article Title:Strong immunogenicity and cross-reactivity of Mycobacterium tuberculosis ESX-5 type VII secretion: encoded PE-PPE proteins predicts vaccine potential.
Reference Detail
Reference ID:1023051
Abstract:The genome of Mycobacterium tuberculosis (Mtb) encodes five type VII secretion systems, ESX-1 to ESX-5, most of which are associated with genes encoding PE/PPE proteins, named after their N-terminal Pro-Glu (PE) or Pro-Pro-Glu (PPE) motifs. Here, we describe the strong T cell immunogenicity of the ESX-5-encoded PE/PPE proteins, which share a large panel of cross-reactive CD4(+) epitopes with substantial numbers of their ESX-5-nonassociated PE/PPE homologs. The immunogenicity of these numerous PE/PPE proteins is dependent on their export by a functional EccD(5), the predicted transmembrane channel of the ESX-5 secretion apparatus. The Mtb ppe25-pe19 mutant deleted for all ESX-5-associated pe and ppe genes, although highly attenuated in immunocompetent mice, remains able to induce immunity against the ESX-5-associated PE/PPE virulence factors, via cross-reactivity with their numerous homologs, and against the ESX-1 virulence factors ESAT-6/CFP-10. The ppe25-pe19 strain is strongly protective against Mtb infection in mice and represents a potential antituberculosis vaccine candidate.
Affiliations:Unité de Régulation Immunitaire et Vaccinologie, Institut Pasteur, Paris, France.
Date:2012
Reference Type:Literature
PubMed ID:22520463
Journal:Cell Host Microbe
Journal Volume:11
Article Pages:352-63
Journal ISSN:1934-6069
Article Chemical List:Antibodies, Bacterial;Bacterial Proteins;Tuberculosis Vaccines
Article MeSH List:Amino Acid Motifs; Amino Acid Sequence; Animals; Antibodies, Bacterial(immunology); Antigenic Variation; Bacterial Proteins(chemistry; genetics; immunology); Bacterial Secretion Systems; Cross Reactions; Female; Humans; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, SCID; Molecular Sequence Data; Mycobacterium tuberculosis(chemistry; genetics; immunology); Protein Transport; Tuberculosis(immunology; microbiology); Tuberculosis Vaccines(chemistry; genetics; immunology)
Curation Last Updated:2013-06-23 20:05:09