Epitopes described in "Protective B-cell epitopes of Francisella tularensis O-polysaccharide in a mouse model of respiratory tularaemia."

Article Authors:Zhaohua Lu; Guillermo Madico; Marly I Roche; Qi Wang; Julia H Hui; Hillary M Perkins; Joseph Zaia; Catherine E Costello; Jacqueline Sharon
Article Title:Protective B-cell epitopes of Francisella tularensis O-polysaccharide in a mouse model of respiratory tularaemia.
Reference Detail
Reference ID:1024410
Abstract:Antibodies to the lipopolysaccharide (LPS) of Francisella tularensis have been shown to be protective against respiratory tularaemia in mouse models, and we have previously described mouse monoclonal antibodies (mAbs) to non-overlapping terminal and internal epitopes of the F. tularensis LPS O-polysaccharide (OAg). In the current study, we used F. tularensis LPS oligosaccharides of defined OAg repeat length as molecular rulers in competition ELISA to demonstrate that the epitope targeted by the terminal OAg-binding mAb FB11 is contained within one tetrasaccharide repeat whereas the epitope targeted by the internal OAg-binding mAb Ab52 spans two tetrasaccharide repeats. Both mAbs conferred survival to BALB/c mice infected intranasally with the F. tularensis type B live vaccine strain and prolonged survival of BALB/c mice infected intranasally with the highly virulent F. tularensis type A strain SchuS4. The protective effects correlated with reduced bacterial burden in mAb-treated infected mice. These results indicate that an oligosaccharide with two OAg tetrasaccharide repeats covers both terminal and internal protective OAg epitopes, which may inform the design of vaccines for tularaemia. Furthermore, the FB11 and Ab52 mAbs could serve as reporters to monitor the response of vaccine recipients to protective B-cell epitopes of F. tularensis OAg.
Affiliations:Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, MA, USA.
Reference Type:Literature
PubMed ID:22486311
Journal Volume:136
Article Pages:352-60
Journal ISSN:0019-2805
Article Chemical List:gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@3d37bb03;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@15d6e15b;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@4e652890;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@534a2e25;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@2b1ba9db;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@252c7ef9
Article MeSH List:Animals; Antibodies, Bacterial(biosynthesis); Antibodies, Monoclonal; Bacterial Load; Bacterial Vaccines(immunology); Disease Models, Animal; Epitopes, B-Lymphocyte(chemistry); Female; Francisella tularensis(immunology; pathogenicity); Mice; Mice, Inbred BALB C; O Antigens(chemistry); Oligosaccharides(chemistry; immunology); Respiratory Tract Infections(immunology; microbiology; prevention & control); Tularemia(immunology; microbiology; prevention & control)
Curation Last Updated:2015-01-18 00:22:42