Epitopes described in "Enhanced humoral and HLA-A2-restricted dengue virus-specific T-cell responses in humanized BLT NSG mice."

Article Authors:Smita Jaiswal; Pamela Pazoles; Marcia Woda; Leonard D Shultz; Dale L Greiner; Michael A Brehm; Anuja Mathew
Article Title:Enhanced humoral and HLA-A2-restricted dengue virus-specific T-cell responses in humanized BLT NSG mice.
Reference Detail
Reference ID:1024418
Abstract:Dengue is a mosquito-borne viral disease of humans, and animal models that recapitulate human immune responses or dengue pathogenesis are needed to understand the pathogenesis of the disease. We recently described an animal model for dengue virus (DENV) infection using humanized NOD-scid IL2r(null) mice (NSG) engrafted with cord blood haematopoietic stem cells. We sought to further improve this model by co-transplantation of human fetal thymus and liver tissues into NSG (BLT-NSG) mice. Enhanced DENV-specific antibody titres were found in the sera of BLT-NSG mice compared with human cord blood haematopoietic stem cell-engrafted NSG mice. Furthermore, B cells generated during the acute phase and in memory from splenocytes of immunized BLT-NSG mice secreted DENV-specific IgM antibodies with neutralizing activity. Human T cells in engrafted BLT-NSG mice secreted interferon- in response to overlapping DENV peptide pools and HLA-A2 restricted peptides. The BLT-NSG mice will allow assessment of human immune responses to DENV vaccines and the effects of previous immunity on subsequent DENV infections.
Affiliations:Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA, USA.
Reference Type:Literature
PubMed ID:22384859
Journal Volume:136
Article Pages:334-43
Journal ISSN:0019-2805
Article Chemical List:Antibodies, Neutralizing;Antibodies, Viral;Dengue Vaccines;HLA-A2 Antigen;RNA, Viral;Interferon-gamma
Article MeSH List:Animals; Antibodies, Neutralizing(blood); Antibodies, Viral(blood); Antibody Specificity; B-Lymphocytes(immunology; virology); Base Sequence; Dengue(immunology; prevention & control; virology); Dengue Vaccines(immunology); Dengue Virus(genetics; immunology); Fetal Tissue Transplantation; HLA-A2 Antigen(metabolism); Humans; Immunity, Humoral; Interferon-gamma(biosynthesis); Liver Transplantation; Mice; Mice, Inbred NOD; Mice, SCID; RNA, Viral(blood; genetics); T-Lymphocytes(immunology; virology); Thymus Gland(transplantation); Viral Load
Curation Last Updated:2015-06-07 20:14:55