Epitopes described in "Enhancement of protective immunity against intracellular bacteria using type-1 polarized dendritic cell (DC) vaccine."

Article Authors:Masato Kono; Yutaro Nakamura; Takafumi Suda; Masato Uchijima; Kunio Tsujimura; Toshi Nagata; Adam S Giermasz; Pawel Kalinski; Hirotoshi Nakamura; Kingo Chida
Article Title:Enhancement of protective immunity against intracellular bacteria using type-1 polarized dendritic cell (DC) vaccine.
Reference Detail
Reference ID:1025333
Abstract:The development of effective vaccine strategies for intracellular bacteria, including tuberculosis, is one of the major frontiers of medical research. Our previous studies showed that dendritic cell (DC) vaccine is a promising approach for eliciting protective immunity against intracellular bacteria. However, it has been reported that standard fully mature DCs show reduced ability to produce IL-12p70 upon subsequent interaction with antigen (Ag)-specific T cells, limiting their in vivo performance for vaccines. Recently, we found that such "DC exhaustion" could be prevented by the presence of IL-4 and IFN- during the maturation of mouse DCs (type-1 polarization), resulting in improved induction of anti-tumor immunity in cancer. Here we show that such type-1 polarized DCs promote dramatic enhancement of protective immunity against an intracellular bacterium, Listeria monocytogenes. Murine bone marrow-derived DCs were cultured and matured with LPS, IL-4 and IFN- (type-1 polarized DCs), and with LPS alone (non-polarized DCs). DCs were loaded with listeriolysin O (LLO) 91-99, H2-K(d)-restricted epitope of L. monocytogenes, and were injected into naïve BALB/c mice intravenously. Type-1 polarized DCs produced significantly higher levels of IL-12p70 than non-polarized DCs in vitro, and this vaccine strongly enhanced LLO 91-99-specific CD8(+) T cells exhibiting epitope-specific cytotoxic activity and IFN- production, leading to significant induction of protective immunity against L. monocytogenes. Type-1 polarized DCs are potential candidates for enhancing protective immunity in the design of effective vaccination strategies against intracellular bacteria.
Affiliations:Second Division, Department of Internal Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan.
Reference Type:Literature
PubMed ID:22365841
Journal Volume:30
Article Pages:2633-9
Journal ISSN:0264-410X
Article Chemical List:Bacterial Toxins;Heat-Shock Proteins;Hemolysin Proteins;Lipopolysaccharides;Interleukin-12;Interleukin-4;hlyA protein, Listeria monocytogenes;Interferon-gamma
Article MeSH List:Animals; Bacterial Toxins(genetics; immunology); CD8-Positive T-Lymphocytes(immunology; metabolism); Cell Polarity(drug effects; immunology); Cells, Cultured; Cytotoxicity, Immunologic; Dendritic Cells(drug effects; immunology; transplantation); Heat-Shock Proteins(genetics; immunology); Hemolysin Proteins(genetics; immunology); Immunity, Cellular; Injections, Intravenous; Interferon-gamma(immunology; pharmacology); Interleukin-12(biosynthesis; immunology); Interleukin-4(immunology; pharmacology); Lipopolysaccharides(immunology; pharmacology); Listeria monocytogenes(immunology); Listeriosis(immunology; prevention & control); Mice; Mice, Inbred BALB C; Vaccination
Curation Last Updated:2015-07-30 20:46:43