Epitopes described in "Human CD4+ T cell response to human herpesvirus 6."

Article Authors:Maria-D Nastke; Aniuska Becerra; Liusong Yin; Omar Dominguez-Amorocho; Laura Gibson; Lawrence J Stern; J Mauricio Calvo-Calle
Article Title:Human CD4+ T cell response to human herpesvirus 6.
Reference Detail
Reference ID:1022991
Abstract:Following primary infection, human herpesvirus 6 (HHV-6) establishes a persistent infection for life. HHV-6 reactivation has been associated with transplant rejection, delayed engraftment, encephalitis, muscular dystrophy, and drug-induced hypersensitivity syndrome. The poor understanding of the targets and outcome of the cellular immune response to HHV-6 makes it difficult to outline the role of HHV-6 in human disease. To fill in this gap, we characterized CD4 T cell responses to HHV-6 using peripheral blood mononuclear cell (PBMC) and T cell lines generated from healthy donors. CD4(+) T cells responding to HHV-6 in peripheral blood were observed at frequencies below 0.1% of total T cells but could be expanded easily in vitro. Analysis of cytokines in supernatants of PBMC and T cell cultures challenged with HHV-6 preparations indicated that gamma interferon (IFN-) and interleukin-10 (IL-10) were appropriate markers of the HHV-6 cellular response. Eleven CD4(+) T cell epitopes, all but one derived from abundant virion components, were identified. The response was highly cross-reactive between HHV-6A and HHV-6B variants. Seven of the CD4(+) T cell epitopes do not share significant homologies with other known human pathogens, including the closely related human viruses human herpesvirus 7 (HHV-7) and human cytomegalovirus (HCMV). Major histocompatibility complex (MHC) tetramers generated with these epitopes were able to detect HHV-6-specific T cell populations. These findings provide a window into the immune response to HHV-6 and provide a basis for tracking HHV-6 cellular immune responses.
Affiliations:Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Reference Type:Literature
PubMed ID:22357271
Journal:J Virol
Journal Volume:86
Article Pages:4776-92
Journal ISSN:1098-5514
Article Chemical List:Antigens, Viral;Cytokines;Epitopes, T-Lymphocyte;HLA-DR1 Antigen;Peptides;Interferon-gamma
Article MeSH List:Antigens, Viral(immunology); CD4-Positive T-Lymphocytes(immunology); Cell Line; Cross Reactions(immunology); Cytokines(metabolism); Epitopes, T-Lymphocyte(immunology); HLA-DR1 Antigen(chemistry; immunology); Haplotypes; Herpesvirus 6, Human(immunology); Humans; Interferon-gamma(metabolism); Leukocytes, Mononuclear(immunology); Peptides(immunology); Protein Multimerization(immunology); Roseolovirus Infections(immunology; virology); T-Lymphocytes(immunology); T-Lymphocytes, Cytotoxic(immunology); Tissue Donors; Viral Load
Curation Last Updated:2016-04-05 23:19:04