Epitopes described in "Nitration of the pollen allergen bet v 1.0101 enhances the presentation of bet v 1-derived peptides by HLA-DR on human dendritic cells."

Article Authors:Anette C Karle; Gertie J Oostingh; Sonja Mutschlechner; Fatima Ferreira; Peter Lackner; Barbara Bohle; Gottfried F Fischer; Anne B Vogt; Albert Duschl
Article Title:Nitration of the pollen allergen bet v 1.0101 enhances the presentation of bet v 1-derived peptides by HLA-DR on human dendritic cells.
Reference Detail
Reference ID:1025136
Abstract:Nitration of pollen derived allergens can occur by NO(2) and ozone in polluted air and it has already been shown that nitrated major birch (Betula verrucosa) pollen allergen Bet v 1.0101 (Bet v 1) exhibits an increased potency to trigger an immune response. However, the mechanisms by which nitration might contribute to the induction of allergy are still unknown. In this study, we assessed the effect of chemically induced nitration of Bet v 1 on the generation of HLA-DR associated peptides. Human dendritic cells were loaded with unmodified Bet v 1 or nitrated Bet v 1, and the naturally processed HLA-DR associated peptides were subsequently identified by liquid chromatography-mass spectrometry. Nitration of Bet v 1 resulted in enhanced presentation of allergen-derived HLA-DR-associated peptides. Both the copy number of Bet v 1 derived peptides as well as the number of nested clusters was increased. Our study shows that nitration of Bet v 1 alters antigen processing and presentation via HLA-DR, by enhancing both the quality and the quantity of the Bet v 1-specific peptide repertoire. These findings indicate that air pollution can contribute to allergic diseases and might also shed light on the analogous events concerning the nitration of self-proteins.
Affiliations:Immunosafety, Non-Clinical Drug Safety, F. Hoffmann-La Roche Ltd, Basel, Switzerland.
Reference Type:Literature
PubMed ID:22348091
Journal:PLoS One
Journal Volume:7
Article Pages:e31483
Journal ISSN:1932-6203
Article Chemical List:Allergens;Antigens, Plant;HLA-DR Antigens;Nitrates;Peptides;Bet v 1 allergen, Betula
Article MeSH List:Air Pollution(adverse effects); Allergens(chemistry; immunology; metabolism); Antigen Presentation(immunology); Antigens, Plant(metabolism); Betula; Dendritic Cells(immunology); HLA-DR Antigens(immunology); Humans; Hypersensitivity(etiology); Nitrates(metabolism); Peptides; Pollen(immunology)
Curation Last Updated:2016-04-04 22:39:11