Epitopes described in "A West Nile virus CD4 T cell epitope improves the immunogenicity of dengue virus serotype 2 vaccines."

Article Authors:Holly R Hughes; Wayne D Crill; Brent S Davis; Gwong-Jen J Chang
Article Title:A West Nile virus CD4 T cell epitope improves the immunogenicity of dengue virus serotype 2 vaccines.
Reference Detail
Reference ID:1022837
Abstract:Flaviviruses, such as dengue virus (DENV) and West Nile virus (WNV), are among the most prevalent human disease-causing arboviruses world-wide. As they continue to expand their geographic range, multivalent flavivirus vaccines may become an important public health tool. Here we describe the immune kinetics of WNV DNA vaccination and the identification of a CD4 epitope that increases heterologous flavivirus vaccine immunogenicity. Lethal WNV challenge two days post-vaccination resulted in 90% protection with complete protection by four days, and was temporally associated with a rapid influx of activated CD4 T cells. CD4 T cells from WNV vaccinated mice could be stimulated from epitopic regions in the envelope protein transmembrane domain. Incorporation of this WNV epitope into DENV-2 DNA and virus-like particle vaccines significantly increased neutralizing antibody titers. Incorporating such potent epitopes into multivalent flavivirus vaccines could improve their immunogenicity and may help alleviate concerns of imbalanced immunity in multivalent vaccine approaches.
Affiliations:Arboviral Diseases Branch, Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Fort Collins, CO 80521, USA
Reference Type:Literature
PubMed ID:22244913
Journal Volume:424
Article Pages:129-37
Journal ISSN:0042-6822
Article Chemical List:Antibodies, Viral;Dengue Vaccines;Epitopes, T-Lymphocyte;West Nile Virus Vaccines
Article MeSH List:Animals; Antibodies, Viral(immunology); Dengue(immunology; prevention & control; virology); Dengue Vaccines(administration & dosage; genetics; immunology); Dengue Virus(genetics; immunology); Epitopes, T-Lymphocyte(genetics; immunology); Humans; Mice; Mice, Inbred C57BL; Vaccination; West Nile Fever(immunology; prevention & control; virology); West Nile Virus Vaccines(administration & dosage; genetics; immunology); West Nile virus(genetics; immunology)
Curation Last Updated:2015-07-30 20:44:59