Epitopes described in "A human antibody recognizing a conserved epitope of H5 hemagglutinin broadly neutralizes highly pathogenic avian influenza H5N1 viruses."

Article Authors:Hongxing Hu; Jarrod Voss; Guoliang Zhang; Philippi Buchy; Teng Zuo; Lulan Wang; Feng Wang; Fan Zhou; Guiqing Wang; Cheguo Tsai; Lesley Calder; Steve J Gamblin; Linqi Zhang; Vincent Deubel; Boping Zhou; John J Skehel; Paul Zhou
Article Title:A human antibody recognizing a conserved epitope of H5 hemagglutinin broadly neutralizes highly pathogenic avian influenza H5N1 viruses.
Reference Detail
Reference ID:1022838
Abstract:Influenza A virus infection is a persistent threat to public health worldwide due to its ability to evade immune surveillance through rapid genetic drift and shift. Current vaccines against influenza A virus provide immunity to viral isolates that are similar to vaccine strains. High-affinity neutralizing antibodies against conserved epitopes could provide immunity to diverse influenza virus strains and protection against future pandemic viruses. In this study, by using a highly sensitive H5N1 pseudotype-based neutralization assay to screen human monoclonal antibodies produced by memory B cells from an H5N1-infected individual and molecular cloning techniques, we developed three fully human monoclonal antibodies. Among them, antibody 65C6 exhibited potent neutralization activity against all H5 clades and subclades except for subclade 7.2 and prophylactic and therapeutic efficacy against highly pathogenic avian influenza H5N1 viruses in mice. Studies on hemagglutinin (HA)-antibody complexes by electron microscopy and epitope mapping indicate that antibody 65C6 binds to a conformational epitope comprising amino acid residues at positions 118, 121, 161, 164, and 167 (according to mature H5 numbering) on the tip of the membrane-distal globular domain of HA. Thus, we conclude that antibody 65C6 recognizes a neutralization epitope in the globular head of HA that is conserved among almost all divergent H5N1 influenza stains.
Affiliations:Unit of Anti-Viral Immunity and Genetic Therapy, Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences,Shanghai, China.
Reference Type:Literature
PubMed ID:22238297
Journal:J Virol
Journal Volume:86
Article Pages:2978-89
Journal ISSN:1098-5514
Article Chemical List:Antibodies, Neutralizing;Antibodies, Viral;Epitopes;Hemagglutinin Glycoproteins, Influenza Virus;hemagglutinin, avian influenza A virus
Article MeSH List:Amino Acid Sequence; Animals; Antibodies, Neutralizing(immunology); Antibodies, Viral(immunology); Conserved Sequence; Epitope Mapping; Epitopes(chemistry; genetics; immunology); Female; Hemagglutinin Glycoproteins, Influenza Virus(chemistry; genetics; immunology); Humans; Influenza A Virus, H5N1 Subtype(chemistry; genetics; immunology); Influenza A virus(chemistry; genetics; immunology); Influenza, Human(immunology; virology); Mice; Mice, Inbred BALB C; Molecular Sequence Data; Neutralization Tests
Curation Last Updated:2015-06-05 03:24:25