Epitopes described in "Potent neutralization of influenza A virus by a single-domain antibody blocking M2 ion channel protein."

Article Authors:Guowei Wei; Weixu Meng; Haijiang Guo; Weiqi Pan; Jinsong Liu; Tao Peng; Ling Chen; Chang-You Chen
Article Title:Potent neutralization of influenza A virus by a single-domain antibody blocking M2 ion channel protein.
Reference Detail
Reference ID:1022671
Abstract:Influenza A virus poses serious health threat to humans. Neutralizing antibodies against the highly conserved M2 ion channel is thought to offer broad protection against influenza A viruses. Here, we screened synthetic Camel single-domain antibody (VHH) libraries against native M2 ion channel protein. One of the isolated VHHs, M2-7A, specifically bound to M2-expressed cell membrane as well as influenza A virion, inhibited replication of both amantadine-sensitive and resistant influenza A viruses in vitro, and protected mice from a lethal influenza virus challenge. Moreover, M2-7A showed blocking activity for proton influx through M2 ion channel. These pieces of evidence collectively demonstrate for the first time that a neutralizing antibody against M2 with broad specificity is achievable, and M2-7A may have potential for cross protection against a number of variants and subtypes of influenza A viruses.
Affiliations:State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Reference Type:Literature
PubMed ID:22164266
Journal:PLoS One
Journal Volume:6
Article Pages:e28309
Journal ISSN:1932-6203
Article Chemical List:Antibodies;Ion Channels;M2 protein, Influenza A virus;Oligonucleotides;Viral Matrix Proteins
Article MeSH List:Animals; Antibodies(chemistry); Antibody Specificity; Camels; Cell Line; Chromatography(methods); Dogs; Enzyme-Linked Immunosorbent Assay(methods); Female; Gene Library; HEK293 Cells; Humans; Influenza A virus(immunology); Ion Channels(chemistry); Kinetics; Mice; Mice, Inbred BALB C; Neutralization Tests; Oligonucleotides(chemistry); Polymerase Chain Reaction(methods); Protein Binding; Protein Structure, Tertiary; Surface Plasmon Resonance; Viral Matrix Proteins(chemistry)
Curation Last Updated:2015-06-05 03:18:42