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| Article Authors: | Guowei Wei; Weixu Meng; Haijiang Guo; Weiqi Pan; Jinsong Liu; Tao Peng; Ling Chen; Chang-You Chen |
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| Article Title: | Potent neutralization of influenza a virus by a single-domain antibody blocking m2 ion channel protein. |
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| Reference ID: | 1022671 |
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| Abstract: | Influenza A virus poses serious health threat to humans. Neutralizing antibodies against the highly conserved M2 ion channel is thought to offer broad protection against influenza A viruses. Here, we screened synthetic Camel single-domain antibody (VHH) libraries against native M2 ion channel protein. One of the isolated VHHs, M2-7A, specifically bound to M2-expressed cell membrane as well as influenza A virion, inhibited replication of both amantadine-sensitive and resistant influenza A viruses in vitro, and protected mice from a lethal influenza virus challenge. Moreover, M2-7A showed blocking activity for proton influx through M2 ion channel. These pieces of evidence collectively demonstrate for the first time that a neutralizing antibody against M2 with broad specificity is achievable, and M2-7A may have potential for cross protection against a number of variants and subtypes of influenza A viruses. |
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| Affiliations: | State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China. |
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| Date: | 2011 |
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| Reference Type: | Literature |
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| PubMed ID: | 22164266 |
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| Journal: | PLoS One |
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| Journal Volume: | 6 |
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| Article Pages: | e28309 |
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| Journal ISSN: | 1932-6203 |
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| Curation Last Updated: | 2013-05-28 22:37:41 |
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