Epitopes described in "CD40L-null NKT cells provide B cell help for specific antibody responses."

Article Authors:Hemangi B Shah; Sunil K Joshi; Mark L Lang
Article Title:CD40L-null NKT cells provide B cell help for specific antibody responses.
Reference Detail
Reference ID:1026088
Abstract:CD1d-binding glycolipids exert potent adjuvant effects on T-dependent Ab responses. The mechanisms include cognate interaction between CD1d-expressing B cells and TCR-expressing Type I CD1d-restricted natural killer T cells (NKTs). However, the critical NKT-derived factors that stimulate B cells are poorly understood. We tested the hypothesis that CD1d-driven CD40L expression by NKT cells influences humoral immunity. Bone marrow chimeras with CD40L(+/+) or CD40L(-/-) NKT cells were immunized with Ag plus CD1d ligand before measuring Ab responses. CD40L(-/-) NKT cells stimulated higher endpoint Ab titers than controls expressing CD40L. In contrast, immunization of CD40L(-/-) mice revealed that CD40L(-/-) NKT cells could not provide B cell help when Th cells lacked CD40L. We report that CD40L(-/-) NKT cells can provide help for Ab production and do so cooperatively with CD40L(+/+) Th cells. We suggest that the manner in which NKT cells provide B cell help is distinct from that of Th cells.
Affiliations:Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, United States.
Reference Type:Literature
PubMed ID:21959330
Journal Volume:29
Article Pages:9132-6
Journal ISSN:0264-410X
Article Chemical List:Antibodies;Antigens, CD1d;CD40 Ligand
Article MeSH List:Animals; Antibodies(blood; immunology); Antibody Formation(immunology); Antigens, CD1d; B-Lymphocytes(immunology); CD40 Ligand(immunology); Lymphocytes, Null(immunology); Mice; Mice, Inbred C57BL; Mice, Knockout; Natural Killer T-Cells(immunology); T-Lymphocytes, Helper-Inducer(immunology)
Curation Last Updated:2016-04-11 21:24:34