Epitopes described in "Lack of IL-7 and IL-15 signaling affects interferon-γ production by, more than survival of, small intestinal intraepithelial memory CD8+ T cells."

Reference
Article Authors:Dmitry Isakov; Amiran Dzutsev; Jay A Berzofsky; Igor M Belyakov
Article Title:Lack of IL-7 and IL-15 signaling affects interferon-γ production by, more than survival of, small intestinal intraepithelial memory CD8+ T cells.
Reference Detail
Reference ID:1025839
Abstract:Survival of antigen-specific CD8(+) T cells in peripheral lymphoid organs during viral infection is known to be dependent predominantly on IL-7 and IL-15. However, little is known about a possible influence of tissue environmental factors on this process. To address this question, we studied survival of memory antigen-specific CD8(+) T cells in the small intestine. Here, we show that 2 months after vaccinia virus infection, B8R(20-27) /H2-K(b) tetramer(+) CD8(+) T cells in the small intestinal intraepithelial (SI-IEL) layer are found in mice deficient in IL-15 expression. Moreover, SI-IEL and lamina propria lymphocytes do not express the receptor for IL-7 (IL-7R/CD127). In addition, after in vitro stimulation with B8R(20-27) peptide, SI-IEL cells do not produce high amounts of IFN- neither at 5 days nor at 2 months postinfection (p.i.). Importantly, the lack of IL-15 was found to shape the functional activity of antigen-specific CD8(+) T cells, by narrowing the CTL avidity repertoire. Taken together, these results reveal that survival factors, as well as the functional activity, of antigen-specific CD8(+) T cells in the SI-IEL compartments may markedly differ from their counterparts in peripheral lymphoid tissues.
Affiliations:Molecular Immunogenetics and Vaccine Research Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Date:2011
Reference Type:Literature
PubMed ID:21928282
Journal:Eur J Immunol
Journal Volume:41
Article Pages:3513-28
Journal ISSN:1521-4141
Article Chemical List:Interleukin-15;Interleukin-7;Interleukin-7 Receptor alpha Subunit;Receptors, Antigen, T-Cell, alpha-beta;Receptors, Interleukin-7;interleukin-7 receptor, alpha chain;Interferon-gamma
Article MeSH List:Animals; CD8-Positive T-Lymphocytes(immunology; metabolism); Female; Immunity, Mucosal(immunology); Immunologic Memory; Interferon-gamma(biosynthesis; immunology); Interleukin-15(immunology; metabolism); Interleukin-7(immunology; metabolism); Interleukin-7 Receptor alpha Subunit(immunology; metabolism); Intestinal Mucosa(immunology; metabolism); Intestine, Small(immunology; metabolism); Lymphocytes(immunology; metabolism); Mice; Mice, Inbred C57BL; Receptors, Antigen, T-Cell, alpha-beta(immunology); Receptors, Interleukin-7(immunology; metabolism); Signal Transduction(immunology); Vaccinia(immunology)
Curation Last Updated:2014-10-03 22:54:08