Epitopes described in "An epitope conserved in orthopoxvirus A13 envelope protein is the target of neutralizing and protective antibodies."

Article Authors:Chungui Xu; Xiangzhi Meng; Bo Yan; Shane Crotty; Junpeng Deng; Yan Xiang
Article Title:An epitope conserved in orthopoxvirus A13 envelope protein is the target of neutralizing and protective antibodies.
Reference Detail
Reference ID:1022385
Abstract:Primary immunization of humans with smallpox vaccine (live vaccinia virus (VACV)) consistently elicits antibody responses to six VACV virion membrane proteins, including A13. However, whether anti-A13 antibody contributes to immune protection against orthopoxviruses was unknown. Here, we isolated a murine monoclonal antibody (mAb) against A13 from a mouse that had been infected with VACV. The anti-A13 mAb bound to recombinant A13 protein with an affinity of 3.4nM and neutralized VACV mature virions. Passive immunization of mice with the anti-A13 mAb protected against intranasal VACV infection. The epitope of the anti-A13 mAb was mapped to a 10-amino acid sequence conserved in all orthopoxviruses, including viriola virus and monkeypox virus, suggesting that anti-A13 antibodies elicited by smallpox vaccine might contribute to immune protection against orthopoxviruses. In addition, our data demonstrates that anti-A13 mAbs are effective for treating orthopoxvirus infection.
Affiliations:Department of Microbiology and Immunology, Univ. of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Reference Type:Literature
PubMed ID:21810533
Journal Volume:418
Article Pages:67-73
Journal ISSN:0042-6822
Article Chemical List:Antibodies, Monoclonal;Antibodies, Neutralizing;Antibodies, Viral;Epitopes, B-Lymphocyte;Viral Envelope Proteins
Article MeSH List:Amino Acid Sequence; Animals; Antibodies, Monoclonal(administration & dosage; immunology; isolation & purification); Antibodies, Neutralizing(administration & dosage; immunology; isolation & purification); Antibodies, Viral(administration & dosage; immunology; isolation & purification); Body Weight; Conserved Sequence; Disease Models, Animal; Epitope Mapping; Epitopes, B-Lymphocyte(genetics; immunology); Immunization, Passive; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Monkeypox virus(genetics); Neutralization Tests; Rodent Diseases(prevention & control); Survival Analysis; Vaccinia(prevention & control); Vaccinia virus(genetics; immunology); Variola virus(genetics); Viral Envelope Proteins(genetics; immunology); Viral Plaque Assay
Curation Last Updated:2015-06-05 02:55:16