|
| Article Authors: | Chungui Xu; Xiangzhi Meng; Bo Yan; Shane Crotty; Junpeng Deng; Yan Xiang |
|---|
| Article Title: | An epitope conserved in orthopoxvirus A13 envelope protein is the target of neutralizing and protective antibodies. |
|---|
|
| Reference ID: | 1022385 |
|---|
| Abstract: | Primary immunization of humans with smallpox vaccine (live vaccinia virus (VACV)) consistently elicits antibody responses to six VACV virion membrane proteins, including A13. However, whether anti-A13 antibody contributes to immune protection against orthopoxviruses was unknown. Here, we isolated a murine monoclonal antibody (mAb) against A13 from a mouse that had been infected with VACV. The anti-A13 mAb bound to recombinant A13 protein with an affinity of 3.4nM and neutralized VACV mature virions. Passive immunization of mice with the anti-A13 mAb protected against intranasal VACV infection. The epitope of the anti-A13 mAb was mapped to a 10-amino acid sequence conserved in all orthopoxviruses, including viriola virus and monkeypox virus, suggesting that anti-A13 antibodies elicited by smallpox vaccine might contribute to immune protection against orthopoxviruses. In addition, our data demonstrates that anti-A13 mAbs are effective for treating orthopoxvirus infection. |
|---|
| Affiliations: | Department of Microbiology and Immunology, Univ. of Texas Health Science Center at San Antonio, San Antonio, TX, USA. |
|---|
| Date: | 2011 |
|---|
| Reference Type: | Literature |
|---|
| PubMed ID: | 21810533 |
|---|
| Journal: | Virology |
|---|
| Journal Volume: | 418 |
|---|
| Article Pages: | 67-73 |
|---|
| Journal ISSN: | 1096-0341 |
|---|
| Curation Last Updated: | 2012-05-31 20:51:58 |
|---|
