Epitopes described in "Epitope mapping of antibodies to VlsE protein of Borrelia burgdorferi in post-Lyme disease syndrome."

Article Authors:Abhishek Chandra; Norman Latov; Gary P Wormser; Adriana R Marques; Armin Alaedini
Article Title:Epitope mapping of antibodies to VlsE protein of Borrelia burgdorferi in post-Lyme disease syndrome.
Reference Detail
Reference ID:1022483
Abstract:The VlsE lipoprotein of Borrelia burgdorferi elicits a strong immune response during the course of Lyme disease. The present study was aimed at characterization of the epitopes of VlsE targeted by the antibody response in patients with post-Lyme disease syndrome, a condition characterized by persisting symptoms of pain, fatigue, and/or neurocognitive impairment despite antibiotic treatment of B. burgdorferi infection. Epitope mapping was carried out using microarrays that contained synthesized overlapping peptides covering the full sequence of VlsE from B. burgdorferi B31. In addition to the previously characterized IR6 region in the variable domain, specific sequences in the N- and C-terminal invariable domains of VlsE were found to be major B cell epitopes in affected patients. The crystal structure of VlsE indicated that the newly described epitopes form a contiguous region in the surface-exposed membrane-proximal part of the monomeric form of the protein.
Affiliations:Department of Neurology and Neuroscience, Weill Cornell Medical College, Cornell University, New York, NY, USA.
Reference Type:Literature
PubMed ID:21778118
Journal:Clin Immunol
Journal Volume:141
Article Pages:103-10
Journal ISSN:1521-6616
Article Chemical List:Antibodies, Bacterial;Antigens, Bacterial;Bacterial Proteins;Epitopes, B-Lymphocyte;Lipoproteins;VlsE protein, Borrelia burgdorferi
Article MeSH List:Adult; Aged; Amino Acid Sequence; Antibodies, Bacterial(blood); Antigens, Bacterial(chemistry; genetics; immunology); Bacterial Proteins(chemistry; genetics; immunology); Borrelia burgdorferi(genetics; immunology); Epitope Mapping; Epitopes, B-Lymphocyte(chemistry; genetics; immunology); Female; Humans; Lipoproteins(chemistry; genetics; immunology); Lyme Neuroborreliosis(immunology; microbiology); Male; Middle Aged; Models, Molecular; Molecular Sequence Data; Protein Array Analysis; Syndrome
Curation Last Updated:2015-12-28 20:02:53