Epitopes described in "A highly conserved neutralizing epitope on group 2 influenza A viruses."

Article Authors:Damian C Ekiert; Robert H E Friesen; Gira Bhabha; Ted Kwaks; Mandy Jongeneelen; Wenli Yu; Carla Ophorst; Freek Cox; Hans J W M Korse; Boerries Brandenburg; Ronald Vogels; Just P J Brakenhoff; Ronald Kompier; Martin H Koldijk; Lisette A H M Cornelissen; Leo L M Poon; Malik Peiris; Wouter Koudstaal; Ian A Wilson; Jaap Goudsmit
Article Title:A highly conserved neutralizing epitope on group 2 influenza A viruses.
Reference Detail
Reference ID:1022349
Abstract:Current flu vaccines provide only limited coverage against seasonal strains of influenza viruses. The identification of V(H)1-69 antibodies that broadly neutralize almost all influenza A group 1 viruses constituted a breakthrough in the influenza field. Here, we report the isolation and characterization of a human monoclonal antibody CR8020 with broad neutralizing activity against most group 2 viruses, including H3N2 and H7N7, which cause severe human infection. The crystal structure of Fab CR8020 with the 1968 pandemic H3 hemagglutinin (HA) reveals a highly conserved epitope in the HA stalk distinct from the epitope recognized by the V(H)1-69 group 1 antibodies. Thus, a cocktail of two antibodies may be sufficient to neutralize most influenza A subtypes and, hence, enable development of a universal flu vaccine and broad-spectrum antibody therapies.
Affiliations:Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Reference Type:Literature
PubMed ID:21737702
Journal Volume:333
Article Pages:843-50
Journal ISSN:1095-9203
Article Chemical List:Antibodies, Monoclonal;Antibodies, Neutralizing;Antibodies, Viral;Antigens, Viral;Epitopes;Hemagglutinin Glycoproteins, Influenza Virus;Influenza Vaccines
Article MeSH List:Amino Acid Sequence; Animals; Antibodies, Monoclonal(immunology; isolation & purification); Antibodies, Neutralizing(immunology; isolation & purification); Antibodies, Viral(immunology; isolation & purification); Antibody Specificity; Antigens, Viral(chemistry; genetics; immunology); Binding Sites, Antibody; Conserved Sequence; Crystallography, X-Ray; Epitopes(immunology); Hemagglutinin Glycoproteins, Influenza Virus(chemistry; genetics; immunology); Humans; Influenza A Virus, H3N2 Subtype(immunology); Influenza A Virus, H7N7 Subtype(genetics; immunology); Influenza A virus(immunology); Influenza Vaccines(immunology); Influenza, Human(immunology; prevention & control; therapy); Mice; Models, Molecular; Molecular Sequence Data; Mutation; Neutralization Tests; Orthomyxoviridae Infections(immunology; prevention & control); Protein Conformation
Curation Last Updated:2015-10-12 20:01:28