Epitopes described in "Shifting hierarchies of interleukin-10-producing T cell populations in the central nervous system during acute and persistent viral encephalomyelitis."

Reference
Article Authors:Shweta S Puntambekar; Cornelia C Bergmann; Carine Savarin; Christopher L Karp; Timothy W Phares; Gabriel I Parra; David R Hinton; Stephen A Stohlman
Article Title:Shifting hierarchies of interleukin-10-producing T cell populations in the central nervous system during acute and persistent viral encephalomyelitis.
Reference Detail
Reference ID:1022084
Abstract:Interleukin-10 (IL-10) mRNA is rapidly upregulated in the central nervous system (CNS) following infection with neurotropic coronavirus and remains elevated during persistent infection. Infection of transgenic IL-10/green fluorescent protein (GFP) reporter mice revealed that CNS-infiltrating T cells were the major source of IL-10, with minimal IL-10 production by macrophages and resident microglia. The proportions of IL-10-producing cells were initially similar in CD8(+) and CD4(+) T cells but diminished rapidly in CD8(+) T cells as the virus was controlled. Overall, the majority of IL-10-producing CD8(+) T cells were specific for the immunodominant major histocompatibility complex (MHC) class I epitope. Unlike CD8(+) T cells, a large proportion of CD4(+) T cells within the CNS retained IL-10 production throughout persistence. Furthermore, elevated frequencies of IL-10-producing CD4(+) T cells in the spinal cord supported preferential maintenance of IL-10 production at the site of viral persistence and tissue damage. IL-10 was produced primarily by the CD25(+) CD4(+) T cell subset during acute infection but prevailed in CD25(-) CD4(+) T cells during the transition to persistent infection and thereafter. Overall, these data demonstrate significant fluidity in the T-cell-mediated IL-10 response during viral encephalitis and persistence. While IL-10 production by CD8(+) T cells was limited primarily to the time of acute effector function, CD4(+) T cells continued to produce IL-10 throughout infection. Moreover, a shift from predominant IL-10 production by CD25(+) CD4(+) T cells to CD25(-) CD4(+) T cells suggests that a transition to nonclassical regulatory T cells precedes and is retained during CNS viral persistence.
Date:2011
Reference Type:Literature
PubMed ID:21525347
Journal:J Virol
Journal Volume:85
Article Pages:6702-13
Journal ISSN:1098-5514
Article Chemical List:Interleukin-2 Receptor alpha Subunit;Interleukin-10
Article MeSH List:Acute Disease; Animals; CD4-Positive T-Lymphocytes(immunology); CD8-Positive T-Lymphocytes(immunology); Central Nervous System(immunology; virology); Chronic Disease; Encephalitis, Viral(immunology; virology); Encephalomyelitis(immunology; virology); Interleukin-10(biosynthesis); Interleukin-2 Receptor alpha Subunit(metabolism); Mice; Mice, Inbred C57BL; Murine hepatitis virus(immunology; pathogenicity); T-Lymphocyte Subsets(immunology)
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