Epitopes described in "Defining the herpes simplex virus-specific CD8+ T cell repertoire in C57BL/6 mice."

Article Authors:Anthony J St Leger; Bjoern Peters; John Sidney; Alessandro Sette; Robert L Hendricks
Article Title:Defining the herpes simplex virus-specific CD8+ T cell repertoire in C57BL/6 mice.
Reference Detail
Reference ID:1021528
Abstract:HSV type 1 (HSV-1) expresses its genes sequentially as immediate early (), early (), leaky late (1), and true late (2), where viral DNA synthesis is an absolute prerequisite only for 2 gene expression. The 1 protein glycoprotein B (gB) contains a strongly immunodominant CD8(+) T cell epitope (gB(498-505)) that is recognized by 50% of both the CD8(+) effector T cells in acutely infected trigeminal ganglia (TG) and the CD8(+) memory T cells in latently infected TG. Of 376 predicted HSV-1 CD8(+) T cell epitopes in C57BL/6 mice, 19 (gB(498-505) and 18 subdominant epitopes) stimulated CD8(+) T cells in the spleens and TG of HSV-1 acutely infected mice. These 19 epitopes identified virtually all CD8(+) T cells in the infected TG that represent all or the vast majority of the HSV-specific CD8(+) TCR repertoire. Only 11 of ∼84 HSV-1 proteins are recognized by CD8(+) T cells, and most (∼80%) are expressed before viral DNA synthesis. Neither the immunodominance of gB(498-505) nor the dominance hierarchy of the subdominant epitopes is due solely to MHC or TCR affinity. We conclude that the vast majority of CD8(+) T cells in HSV-1 acutely infected TG are HSV specific, that HSV-1 and 1 proteins that are expressed before viral DNA synthesis are favored targets of CD8(+) T cells, and that dominance within the TCR repertoire is likely due to the frequency or expansion and survival characteristics of CD8(+) T cell precursors.
Reference Type:Literature
PubMed ID:21357536
Journal:J Immunol
Journal Volume:186
Article Pages:3927-33
Journal ISSN:0022-1767
Article Chemical List:Epitopes, T-Lymphocyte;Immunodominant Epitopes;Receptors, Antigen, T-Cell
Article MeSH List:Acute Disease; Animals; CD8-Positive T-Lymphocytes(immunology; metabolism; virology); Cell Line; Cells, Cultured; Epitopes, T-Lymphocyte(immunology; isolation & purification); Female; Herpes Simplex(immunology; pathology; virology); Herpesvirus 1, Human(growth & development; immunology); Immunodominant Epitopes(biosynthesis; immunology; isolation & purification); Keratitis, Herpetic(immunology; metabolism; virology); Mice; Mice, Inbred C57BL; Receptors, Antigen, T-Cell(biosynthesis); Trigeminal Ganglion(immunology; metabolism; virology); Virus Latency(immunology)
Curation Last Updated:2015-07-30 20:39:02