Epitopes described in "Identification of immunoreactive epitopes of the Porphyromonas gingivalis heat shock protein in periodontitis and atherosclerosis."

Article Authors:J Choi; S-Y Lee; K Kim; B-K Choi
Article Title:Identification of immunoreactive epitopes of the Porphyromonas gingivalis heat shock protein in periodontitis and atherosclerosis.
Reference Detail
Reference ID:1021613
Abstract:BACKGROUND AND OBJECTIVE: Heat shock protein 60 (HSP60) of Porphyromonas gingivalis, a major periodontal pathogen, might be a trigger molecule linking infectious periodontitis and autoimmune atherosclerosis. The aim of this study was to identify the peptide specificity of anti-P. gingivalis HSP60 monoclonal antibodies and their cross-reactivity with bacterial and human HSPs. Their specific immunoreactivity to periodontal or atherosclerotic lesions was also investigated. METHODS: Twenty patients with chronic periodontitis and 20 atherosclerosis patients who had undergone surgical intervention for atheromatous plaques with evidence of ongoing periodontal disease, were selected. Synthetic peptide 19 ((TLVVNRLRGSLKICAVKAPG)-specific T-cell lines were established from inflamed gingiva and atheromatous plaque and the phenotypes and cytokine profiles were characterized. RESULTS: Thirty per cent of periodontitis patients and 100% of atherosclerosis patients reacted positively to cross-reactive peptide 19 from both P. gingivalis and human HSP60. The peptide 19-specific T-cell lines demonstrated the phenotype characteristic of helper T cells (CD4(+)) but did not express CD25 or FOXP3. The interleukin-10 levels were elevated significantly in the peptide 19 T-cell line. CONCLUSION: Synthetic peptide 19 of P. gingivalis HSP60 is an immunoreactive epitope in the periodontitis-atherosclerosis axis.
Affiliations:Department of Periodontology, School of Dentistry, Pusan National University, Yangsan City, South Korea. jrapa@pusan.ac.kr
Reference Type:Literature
PubMed ID:21241301
Journal:J Periodontal Res
Journal Volume:46
Article Pages:240-5
Journal ISSN:1600-0765
Article Chemical List:Antibodies, Monoclonal;Chaperonin 60;Cytokines;Epitopes;Epitopes, T-Lymphocyte;FOXP3 protein, human;Forkhead Transcription Factors;Interleukin-2 Receptor alpha Subunit;Peptide Fragments;Interleukin-10
Article MeSH List:Antibodies, Monoclonal(immunology); Atherosclerosis(microbiology); Blotting, Western; CD4-Positive T-Lymphocytes(immunology); Cell Line; Chaperonin 60(immunology); Chromatography, High Pressure Liquid; Chronic Periodontitis(microbiology); Cross Reactions(immunology); Cytokines(analysis); Epitopes(analysis; immunology); Epitopes, T-Lymphocyte(analysis; immunology); Forkhead Transcription Factors(analysis); Gingivitis(immunology); Humans; Immunoblotting; Immunophenotyping; Interleukin-10(analysis); Interleukin-2 Receptor alpha Subunit(analysis); Peptide Fragments(immunology); Plaque, Atherosclerotic(microbiology); Porphyromonas gingivalis(immunology); T-Lymphocytes, Helper-Inducer(immunology)
Curation Last Updated:2015-01-27 22:00:40