Epitopes described in "Involvement of CD8+ T cell-mediated immune responses in LcrV DNA vaccine induced protection against lethal Yersinia pestis challenge."

Article Authors:Shixia Wang; Jon D Goguen; Fusheng Li; Shan Lu
Article Title:Involvement of CD8+ T cell-mediated immune responses in LcrV DNA vaccine induced protection against lethal Yersinia pestis challenge.
Reference Detail
Reference ID:1022422
Abstract:Yersinia pestis (Y. pestis) is the causative pathogen of plague, a highly fatal disease for which an effective vaccine, especially against mucosal transmission, is still not available. Like many bacterial infections, antigen-specific antibody responses have been traditionally considered critical, if not solely responsible, for vaccine-induced protection against Y. pestis. Studies in recent years have suggested the importance of T cell immune responses against Y. pestis infection but information is still limited about the details of Y. pestis antigen-specific T cell immune responses. In current report, studies are conducted to identify the presence of CD8+ T cell epitopes in LcrV protein, the leading antigen of plague vaccine development. Furthermore, depletion of CD8+ T cells in LcrV DNA vaccinated Balb/C mice led to reduced protection against lethal intranasal challenge of Y. pestis. These findings establish that an LcrV DNA vaccine is able to elicit CD8+ T cell immune responses against specific epitopes of this key plague antigen and that a CD8+ T cell immune response is involved in LcrV DNA vaccine-elicited protection. Future studies in plague vaccine development will need to examine if the presence of detectable T cell immune responses, in particular CD8+ T-cell immune responses, will enhance the protection against Y. pestis in higher animal species or humans.
Affiliations:Laboratory of Nucleic Acid Vaccines, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Reference Type:Literature
PubMed ID:21199697
Journal Volume:29
Article Pages:6802-9
Journal ISSN:0264-410X
Article Chemical List:Antigens, Bacterial;Bacterial Vaccines;Epitopes;LcrV protein, Yersinia;Pore Forming Cytotoxic Proteins;Vaccines, DNA;Interferon-gamma
Article MeSH List:Animals; Antigens, Bacterial(genetics; immunology); Bacterial Vaccines(administration & dosage; immunology); CD4-Positive T-Lymphocytes(immunology); CD8-Positive T-Lymphocytes(immunology); Enzyme-Linked Immunospot Assay; Epitopes(immunology); Female; Immunity, Cellular; Interferon-gamma(analysis); Lethal Dose 50; Mice; Mice, Inbred BALB C; Plague(immunology; prevention & control); Pore Forming Cytotoxic Proteins(genetics; immunology); Vaccination; Vaccines, DNA(administration & dosage; immunology); Yersinia pestis(immunology; pathogenicity)
Curation Last Updated:2015-07-30 20:40:50